选择性β_3受体激动剂对3T3-L1前体脂肪细胞分化的影响及机制  

Effect of β_3-adrenocptor agonist BRL37344 on 3T3-L1 preadipocyte differentiation and its mechanism

作　　者：张华[1,2] 边德志[2] 张梅[2] 张艳红[2] 李丹丹[2] 班博[2] 

机构地区：[1]天津医科大学,天津300070 [2]济宁医学院附属医院

出　　处：《山东医药》2013年第12期15-17,I0002,共4页Shandong Medical Journal

基　　金：山东省自然科学基金资助项目(ZR2009CZ006)

摘　　要：目的探讨选择性β3受体激动剂BRL37344对3T3-L1前体脂肪细胞分化的影响及ERK1/2分子磷酸化在此过程中的作用。方法体外培养3T3-L1前体脂肪细胞,不同药物干预一定时间后裂解细胞收集蛋白,应用Western blot法检测PPARγ、ERK1/2及pERK1/2蛋白表达。结果 10-7mol/L BRL37344可以显著提高3T3-L1前体脂肪细胞内ERK1/2分子的磷酸化水平,下调PPARγ表达。部分阻断BRL37344引起的ERK1/2磷酸化可以恢复PPARγ表达。结论 10-7mol/L BRL37344引起的ERK1/2长时间高水平磷酸化在抑制3T3-L1细胞分化过程中起着十分重要的作用。Objective To study the effect of BRL37344 on 3T3-L1 preadipocyte differentiation and the role of ERK1/ 2 phosphorylation in this process. Methods 3T3-L1 preadipocytes were cultured in vitro with different drugs, Western blot analysis for PPARγ, phospho- and total ERK1/2 was performed by using cell lysates. Results 10-7 mol/L BRL37344 could significantly increase ERK1/2 molecule phosphorylation levels in 3T3-L1 preadipocyte and down-regulate the expression of PPARγ; partial blockade of phosphorylation of ERK1/2 induced by BRL37344 could restore PPAR3, ex- pression. Conclusion The long time and high level phosphorylation of ERK1/2 signaling pathway trigged by BRL37344 plays an important role in the process of inhibition of 3T3-L1 preadipocyte differentiation.

关 键 词：肥胖 选择性β3受体激动剂 PERK1/2 3T3-L1前体脂肪细胞 

分 类 号：R589.2[医药卫生—内分泌]