复方绞股蓝软胶囊降血脂作用机制研究  

Study on the Mechanism of Compound Gynostemma Pentaphyllum Soft Capsule for Hypolipidemic Effects

作　　者：黄晓飞[1] 卢锟刚[1] 宋成武[1] 陈桂林[1] 余尚工[1] 方念伯[1] 

机构地区：[1]湖北中医药大学中药资源与中药复方教育部重点实验室,武汉430065

出　　处：《数理医药学杂志》2013年第2期197-200,共4页Journal of Mathematical Medicine

基　　金：湖北大顶山制药有限公司降脂胶囊委托开发项目

摘　　要：目的:研究复方绞股蓝软胶囊降血脂作用机制。方法:采用高脂饲料喂养雄性昆明种小鼠4w,复制实验性高脂血症小鼠模型。连续灌胃复方绞股蓝软胶囊内容物(剂量为345mg.kg-1.d-1)4w,测定小鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白-胆固醇(HDL-C)的含量。同时采用逆转录-聚合酶链式反应技术(RT-PCR)检测肝脏中与脂代谢相关基因:载脂蛋白AI(ApoAI)、胆固醇7α-羟化酶(Cyp7α1)、3-羟-3-甲基戊二酸单酰辅酶A还原酶(Hmgcr)及固醇调控元件结合转录因子2(Srebf2)mR-NA的相对表达量。并对肝、肾、脾等脏器进行病理学检查。结果:与高脂模型组相比较,复方给药组小鼠血清TC、TG含量显著性降低(P<0.01),HDL-C含量显著性升高(P<0.01),小鼠肝脏病变情况较高脂模型组显著性好转。同时小鼠肝脏中ApoAI、Cyp7α1基因mRNA相对表达量显著性上调(P<0.01)、Hmgcr及Srebf2基因mRNA的相对表达量显著下调(P<0.01)。结论:复方绞股蓝软胶囊具有较好的降血脂作用,其降血脂作用机制是对肝脏中与脂代谢相关基因ApoAI、Cyp7α1、Hmgcr及Srebf2的表达调控有关。Objective： To investigate the mechanism of compound Gynostemma pentaphyllum soft capsule（CGS） for hypolipidemic effects.Methods： Hyperlipidemia mice model was established with a high-fat diet for 4 weeks.Then the compound group mice was given CGS（345mg·kg-1·d-1） intragastrically for 4 weeks consecutive days.The effects of CGS on serum levels of total cholesterol（TC）,triglyceride（TG）,high density lipoprotein cholesterol（HDL-C） were measured respectively,as well as the detection of liver apolipoprotein AI（ApoAI）,3-hydroxy-3-methylglutaryl coenzyme A reductase（Hmgcr）,cholesterol 7α1-hydroxylase（Cyp7α1）,sterol regulatory element binding transcription factor 2（Srebf2） mRNA expressions was performed by reverse transcriptase polymerase chain reaction（RT-PCR）.The pathological changes of liver tissues stained with Hematoxylin-eosin（HE） were observed under light microscope.Results： Compared with the hyperlipidemia model group mice,the CGS could significantly decreased the level of serum TC,TG and remarkably increased the concentration of serum HDL-C in the hyperlipemia mice（P0.01）,and it had significantly effect on up-regulation of ApoAI and Cyp7α1 mRNAs,down-regulation of Hmgcr and Srebf2 mRNAs（P0.01）.Conclusion： The CGS showed potent hypolipidemic effect,and the mechanism of hypolipidemic effect was probably regulation the mRNAs expression of four key cholesterol metabolism genes in liver.

关 键 词：软胶囊 高脂血症 机制 RT-PCR 

分 类 号：R965[医药卫生—药理学]