Electroacupuncture diminishes P2X_2 and P2X_3 purinergic receptor expression in dorsal root ganglia of rats with visceral hypersensitivity  

作　　者：Zhijun Weng Luyi Wu Yuan Lu Lidong Wang Linying Tan Ming Dong Yuhu Xin 

机构地区：[1]Shanghai Academy of Traditional Chinese Medicine [2]Fudan University Shanghai Cancer Center

出　　处：《Neural Regeneration Research》2013年第9期802-808,共7页中国神经再生研究（英文版）

基　　金：funded by the National Natural Science Foundation of China,No.30973783;the Shanghai Municipal Scientific Committee Project,No.11ZR1434300;the Key Subject Program of State Administration of Traditional Chinese Medicine

摘　　要：Electroacupuncture at Shangjuxu （ST37） and Tianshu （ST25） can improve visceral hypersensitivity in rats. Colorectal distension was used to establish a rat model of chronic visceral hypersensitivity. Immunohistochemistry was used to detect P2X2 and P2X3 receptor expression in dorsal root ganglia from rats with chronic visceral hypersensitivity. Results demonstrated that abdominal withdrawal reflex scores obviously increased following establishment of the model, indicating visceral hypersensitivity. Simultaneously, P2X2 and P2X3 receptor expression increased in dorsal root ganglia. After bilateral electroacupuncture at Shangjuxu and Tianshu, abdominal withdrawal reflex scores and P2X2 and P2X3 receptor expression decreased in rats with visceral hypersensitivity. These results indicated that electroacupuncture treatment improved visceral hypersensitivity in rats with irritable bowel syndrome by reducing P2X2 and P2X3 receptor expression in dorsal root ganglia.Electroacupuncture at Shangjuxu （ST37） and Tianshu （ST25） can improve visceral hypersensitivity in rats. Colorectal distension was used to establish a rat model of chronic visceral hypersensitivity. Immunohistochemistry was used to detect P2X2 and P2X3 receptor expression in dorsal root ganglia from rats with chronic visceral hypersensitivity. Results demonstrated that abdominal withdrawal reflex scores obviously increased following establishment of the model, indicating visceral hypersensitivity. Simultaneously, P2X2 and P2X3 receptor expression increased in dorsal root ganglia. After bilateral electroacupuncture at Shangjuxu and Tianshu, abdominal withdrawal reflex scores and P2X2 and P2X3 receptor expression decreased in rats with visceral hypersensitivity. These results indicated that electroacupuncture treatment improved visceral hypersensitivity in rats with irritable bowel syndrome by reducing P2X2 and P2X3 receptor expression in dorsal root ganglia.

关 键 词：neural regeneration acupuncture and moxibustion P2X2 P2X3 visceral hypersensitivity irritablebowel syndrome ELECTROACUPUNCTURE P2 purinergic receptors abdominal withdrawal reflex scoresacupuncture and moxibustion peripheral nerve injury grants-supported paper photographscontaining paper neuroregeneration 

分 类 号：R245[医药卫生—针灸推拿学]