兔迷走神经压迫后对血浆血管紧张素Ⅱ及其1型受体mRNA表达水平的检测  

The detection of the levels of plasma AngⅡand AT1R mRNA expression after the compression of rabbit's vagus nerve

作　　者：呼格吉乐[1] 董莉[1] 张彩虹[1] 渠志臻[1] 高乃康[2] 

机构地区：[1]内蒙古医学院附属医院检验科,内蒙古呼和浩特010050 [2]内蒙古医学院附属医院神经外科,内蒙古呼和浩特010050

出　　处：《国际检验医学杂志》2013年第5期515-516,共2页International Journal of Laboratory Medicine

基　　金：内蒙古医学院附属医院重大课题资助项目(NYFYZD2006003)

摘　　要：目的通过手术建立兔颈总动脉对迷走神经的持续压迫的动物模型,检测血浆血管紧张素Ⅱ(AngⅡ)水平及其1型受体(AT1R)mRNA在心肌中的表达水平。方法构建手术组动物模型,将25只新西兰兔的迷走神经与颈部结缔组织固定,造成颈总动脉对迷走神经的持续压迫。同时将25只未经手术处理的新西兰兔作为对照组。采用放射免疫法检测两组新西兰兔血浆AngⅡ的水平,用RT-PCR法检测两组新西兰兔心肌AT1RmRNA的表达水平。结果手术组兔血浆AngⅡ水平和心肌中AT1RmRNA的表达水平均高于对照组(P<0.05)。结论兔颈总动脉对迷走神经的持续压迫会刺激左侧颈部迷走神经,导致兔血浆AngⅡ和心肌AT1RmRNA表达水平的增高。Objective To establish an animal model by fixing vagus nerve with connective tissue to make an cross-compression of vagus nerve and arteria carotis,and then to detect the levels of AngⅡand the mRNA expression of AT1R.Methods As the surgery group,25New Zealand rabbits＇vagus nerves were fixed with connective tissue to make persistent compression of vagus nerve by arteria carotis.Radioimmunoassay was applied to detect AngⅡlevel and RT-PCR to detect the expression of AT1RmRNA.Meanwhile 25New Zealand rabbits without surgical treatment were used as controls.Results The level of plasma AngⅡand myocardial AT1RmRNA expression were enhanced significantly compared with controls.Conclusion The persistent compression from arteria carotis to the demyelinated vagus nerve of left cervical part could enhance the levels of AngⅡand mRNA of AT1R.

关 键 词：迷走神经 血管紧张素Ⅱ 受体 血管紧张素 1型 兔 

分 类 号：R544.1[医药卫生—心血管疾病]