矮丛蓝莓茎段组织的培养  

Study on tissue culture of Vaccinium angustifolium stem segment

作　　者：王新苗 张淑华 索云成 刘婧怡 齐安国[2] 

机构地区：[1]伊春林业学校,黑龙江伊春153000 [2]河南科技学院,河南新乡453003

出　　处：《河南科技学院学报（自然科学版）》2013年第1期23-27,共5页Journal of Henan Institute of Science and Technology(Natural Science Edition)

基　　金：辽宁省教育厅资助项目(2009B157)

摘　　要：以2种矮丛蓝莓的茎段为外植体,进行不定芽的诱导和成苗的研究.筛选出最佳的灭菌方式为体积分数为75%的乙醇30 s+质量分数为0.1%的HgCl28 min;在WPM培养基中附加不同种类、浓度的激素,研究腋芽萌发、生长、增殖的情况.结果表明:6-BA对芽生长有促进作用;6-BA与ZT同时使用,可提高芽的增殖系数;在1/2 MS+IBA培养基中添加AC时,生根效果最好.The induction of adventitious buds from stem segment and raising plantlets in vitro were studied with two Vaccinium angustifoliums.The optimal sterilizing treatment for the stem with axillary bud was 75% alcohol 30 s＋0.1% HgCl2 8 min;The culture medium is WPM supplemented with different kinds and concentrations hormone,study on the conditions of axillary buds germinate,growth and proliferation.The result indicates that 6-BA can promote the buds grow;The multiplication can improve for buds with 6-BA and ZT;The best rooting effect is the 1/2 MS＋IBA supplemented with AC.

关 键 词：矮丛蓝莓 茎段 组织培养 

分 类 号：S663.9[农业科学—果树学]