脊髓Toll样受体4在慢性瘙痒中的作用研究  

Role of spinal Toll-like receptor 4 in chronic pruritus

作　　者：孔敏[1] 周芳[1] 党秀静[1] 武静茹[1] 

机构地区：[1]徐州医学院/江苏省麻醉学重点实验室,江苏徐州221002

出　　处：《重庆医学》2013年第9期961-963,共3页Chongqing medicine

基　　金：国家自然科学基金青年基金项目(30900417);徐州市科技计划项目(XM12B017)

摘　　要：目的研究脊髓Toll样受体4(TLR4)在重复使用噁唑酮引起的慢性瘙痒中的作用。方法选择雄性昆明小鼠152只,先随机分为3组:对照组、丙酮溶媒组和噁唑酮组,每组40只,每组再分为首次刺激前、激发0、4、9、11d5个亚组,按模型制作方法在相应时间点涂抹相应试剂,记录激发后30min行为学后处死小鼠,取脊髓颈膨大采用Western blot法检测TLR4表达水平。在确定噁唑酮致慢性瘙痒后9d小鼠脊髓TLR4表达水平接近高峰的基础上,为研究腹腔注射表没食子儿茶素没食子酸酯(EGCG)的作用,小鼠随机分为4组(n=8),丙酮溶媒组(A组)、丙酮溶媒+EGCG组(A+E组),噁唑酮组(O组)和噁唑酮+EGCG组(O+E组)。各组在第9天激发后8h开始腹腔注射EGCG(40mg/kg)或生理盐水,每8小时1次直到第11天激发前,共计6次,最后一次给药后立即激发,观察激发后30min内行为学改变,之后处死小鼠采用Western blot法检测颈段脊髓TLR4表达。结果噁唑酮组在激发后各时间点搔抓行为明显多于对照组和丙酮溶媒组(P<0.05),且脊髓TLR4表达水平与搔抓行为呈正相关(r=0.947)。结论腹腔注射EGCG可以减少脊髓TLR4表达水平与搔抓行为。脊髓TLR4参与重复使用噁唑酮引起的慢性瘙痒过程。Objective To investigate the role of spinal Toll-like receptor 4（TLR4） in chronic pruritix induced by repeated administration of oxazolone.Methods 152 male kunming mice were selected and divided into 3 groups with 40 mice in each group：the contol group（C）,acetone group（A） and oxazolone group（O）,and each group was redivided into 5 sub-groups including before first stimulation,on 0,4,9 11 d stimulation sub-groups.Mice in each group were treated with nothing,acetone or oxazolone.The scratching behaviors were observed for 30 min after every stimulation,then the cervical enlargement of spinal cord of mice was used to test the expression of TLR4.To investigate the effect of intraperitonieal injection of EGCG（40 mg/kg）,mice were randomly divided into four groups：acetone group（A）,acetone＋EGCG group（A＋E）,oxazolone group（O） and oxazolone＋EGCG group（O＋E）.EGCG was intraperitoneally injected from 8 h after stimulation on 9 d,one time/8 h,for 6 times to the beginning of the last stimulation on 11 d.The TLR4 expression from mice was detected with Western blot assaying 30 min after the last stimulation on 11 d.Results From the first stimulation,the scratching behaviors at various time points in the group O were significantly more than those in the group C and A（P0.05）,and the spinal TLR4 expression was positively correlated with the scratching behaviors（r=0.947）.Conclusion Intraperitoneal injection of EGCG could reduce the spinal TLR4 expression and the scratching behaviors.Spinal TLR4 mediates the chronic pruritis induced by repeated administration of oxazolone.

关 键 词：噁唑酮 瘙痒 TOLL样受体4 

分 类 号：R758.25[医药卫生—皮肤病学与性病学]