利多卡因对幼鼠小肠缺血-再灌注肠组织损伤的影响  

Influence of lidocaine on intestinal tissue injury induced by intestinal ischemia/reperfusion in young rats

作　　者：庄立峰[1] 林春榕[2] 潘云[1] 鲁智英[1] 周雨[1] 黄勇[1] 姚瑶[1] 吴学东[1] 

机构地区：[1]大理学院附属医院暨临床医学研究中心,云南大理671000 [2]大理学院基础医学院生物化学与分子生物学教研室,云南大理671000

出　　处：《重庆医学》2013年第9期968-970,共3页Chongqing medicine

基　　金：云南省自然科学基金资助项目(2009ZC121M)

摘　　要：目的探讨利多卡因对幼鼠小肠缺血-再灌注(I/R)肠组织损伤的影响。方法将48只4周龄SD幼鼠随机分成3组,每组16只。假手术组仅暴露腹腔脏器,不作任何干预;I/R组,再灌注前经股静脉注射生理盐水0.5mL;利多卡因干预组再灌注前经股静脉注射利多卡因2mg/kg。于再灌注后30、60、90、120min每组各处死4只幼鼠取小肠组织。比较3组幼鼠小肠组织的病理学形态和Chiu′s评分。结果 I/R组和利多卡因干预组小肠组织均发生了明显的炎症病理改变,并随再灌注时间延长而呈现加重的趋势;I/R组和利多卡因干预组各时间点Chiu′s评分均高于假手术组,而I/R组高于利多卡因干预组(P<0.05),其中两组评分均以再灌注90min时为最高。结论幼鼠肠I/R所致肠组织病理损伤呈现随再灌注时间延长而加重的趋势,但利多卡因在一定程度上减轻了I/R所致的肠组织损伤。Objective To explore the influence of lidocaine on intestinal ischemia reperfusion（I/R） injury in young rats.Methods 48 SD rats at 4 weeks old were randomly divided into the sham operated group as control,I/R group and the lidocaine intervention group（Lid）.The abdominal cavity was exposed in the sham group without any intervention.The I/R group was injected with normal saline 0.5 mL via femoral vein before reperfusion and the Lid group was injected with lidocaine 2 mg/kg via femoral vein before reperfusion.4 rats in each group were killed at the time points of 30,60,90,120 min for taking the intestinal tissue specimens.The pathological morphology of the intestinal tissue and the Chiu’s scores were compared among 3 groups.Results The obvious inflammatory changes of intestinal tissue occurred in the I/R group and the Lid group,and showed the aggravating trend with the extending of reperfusion time.The Chiu’s scores at various time points in the I/R group and the Lid group were higher than those in the sham operated group,while the I/R group was higher than the Lid group（P0.05）,among them,which at 90 min reperfusion in these two groups was highest.Conclusion The pathological intestinal injury caused by I/R in young rat shows the aggravating trend with the reperfusion time extending and can be mitigated by lidocaine in a certain degree.

关 键 词：缺血-再灌注损伤 肠组织 利多卡因 幼鼠 

分 类 号：R614[医药卫生—麻醉学]