氮和硅对三角褐指藻细胞增殖的交互作用研究  

Interactive Effects of Nitrogen and Silicon on Cell Proliferation in Marine Diatom Phaeodactylum tricornutum

作　　者：蔡卓平[1] 段舜山[2] 朱红惠[1] 

机构地区：[1]广东省微生物研究所,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东省华南应用微生物重点实验室-省部共建国家重点实验室培育基地,广东广州510070 [2]暨南大学水生生物研究中心,广东广州510632

出　　处：《水产科学》2013年第3期125-129,共5页Fisheries Science

基　　金：国家自然科学基金委(NSFC)-广东联合基金重点项目(U1133003);国家自然科学基金资助项目(41176104,31070103);广东省自然科学基金重点项目(10251007002000001);广东省科技攻关项目(2011B090400613)

摘　　要：设置氮质量浓度和硅质量浓度双因子试验,探讨其对三角褐指藻生长的影响及对藻细胞增殖的交互作用。结果发现,氮质量浓度对三角褐指藻生长有极显著的影响。不论是在低硅质量浓度,还是高硅质量浓度下,与高氮处理相比较,缺氮处理下藻细胞不能存活,低氮处理下藻细胞生长受严重抑制。试验结束时,低硅质量浓度下缺氮、低氮和高氮处理的藻细胞密度分别为0、5.0×105个/mL和7.37×106个/mL。硅质量浓度同样对三角褐指藻的细胞密度产生显著性影响,高硅质量浓度下的藻细胞密度显著高于低硅质量浓度下的藻细胞密度。双因素方差分析结果表明,试验前期,氮和硅对三角褐指藻细胞密度的交互作用不明显(P>0.05);但试验后期,氮和硅对三角褐指藻细胞密度有显著性的交互作用(P<0.05)。In this study, marine diatom Phaeodactylum tricornutum was cultured in different concentrations of nitrogen and silicon, and the cell density and specific growth rate were estimated under laboratory condition to evaluate the interactive effects of nitrogen and silicon on cell proliferation by two-factor ANOVA. The results showed that no survival cells were observed in the depleted nitrogen condition, and poor growth was found in the low nitrogen concentration, as compared to high nitrogen level, indicating that the growth of the alga was significantly affected by nitrogen concentration, either in low or high silicon concentrations. In the low level of silicon, the cell densities were found to be 0 in depleted nitrogen, 5.0 ×10^5 cells/mL in low nitrogen level and 7.37 ×10^6 cells/mL in high level of nitrogen at the end of the experiments. Also, the cell density of the diatom was significantly affected by silicon concentration, with restricted cell density in low silicon level. The two-factor ANOVA revealed that there were no significant interactive effects of nitrogen and silicon on the cell density of the diatom P. tricornutum in first 3;5 day growth stage （P〉0.05） ; whereas the interactive effects became significant in the late growth stage （P〈0.05）.

关 键 词：氮 硅 三角褐指藻 交互作用 

分 类 号：S963.213[农业科学—水产养殖]