青蟹呼肠孤病毒对拟穴青蟹的致病性研究  

The Pathogenicity of Mud Crab Reovirus(MCRV)to Green Crab Scyllaparamamosain

作　　者：蔡小辉[1] 彭银辉[1] 刘旭佳[1] 游出超[1] 苏琼[1] 聂振平[1] 董兰芳[1] 杨志会 王志成[1] 

机构地区：[1]广西海洋研究所广西海洋生物技术重点实验室,广西北海536000 [2]邢台市畜牧水产局,河北邢台054000

出　　处：《水产科学》2013年第3期130-135,共6页Fisheries Science

基　　金：广西自然科学基金资助项目(桂科自2010GXNSFA013079);广西科技攻关资助项目(桂科攻1114012-11)

摘　　要：2008—2012年广西海洋研究所海水增养殖基地暂养的拟穴青蟹膏蟹出现"嗜睡"等典型的青蟹呼肠孤病毒病症状。采用套式PCR检测濒死膏蟹感染青蟹呼肠孤病毒阳性率为100%,经Blastn比对扩增序列与青蟹呼肠孤病毒基因(HQ414137.1)相似度达98%,属于青蟹呼肠孤病毒(JX213634)。病毒粗提液人工回感后发现,攻毒1h,青蟹血淋巴中即可检测到青蟹呼肠孤病毒;攻毒后第4～6d,出现死亡高峰,第10d,累积死亡率达到100%。血清酶指标中碱性磷酸酶、谷丙转氨酶和谷草转氨酶活力明显高于对照组,而与免疫相关的过氧化物酶、超氧化物歧化酶活力低于对照组;肝胰腺、鳃和肌肉组织切片均呈现显著病理坏死损伤。青蟹呼肠孤病毒是此次拟穴青蟹膏蟹死亡的主要原因。The green crab （Scylla pararnamosain） held in the mariculture base of Guangxi Institute of Oceanology showed the typical ＇sleeping disease＇ symptom of mud crab reovirus（MCRV）. In this study, dying green crab collected in 2012 were detected for MCRV by the nest-PCR with the positive rate of 100;, and the amplified sequence had the maximal identity of 98; to MCRV segment 11（HQ414137.1） by Blast alignment, belonging to MCRV（JX213634）. The artificial infection with the virus crude extracts revealed that the MCRV was detected in the hemolymph of the green crab by nest-PCR 1 h post-infection. The peak mortality was observed on the 4-6th days, with cumulative mortality of 100; 10 days after injection. The activities of glutamic-pyruvic transaminase, glutamic-oxaloacetic transaminase and alkaline phosphatase were significantly higher in the treatment groups than in the control group, while the activities of superoxidase dimutase and phenoloxidase related to immunity were found to be decreased in MCRV--infected crabs which showed the pathological necrosis in the hepatopancreas, gills and muscle. The mud crab reovirus is found to be the primary cause for the death of green crab.

关 键 词：拟穴青蟹 青蟹呼肠孤病毒 血清酶指标 组织病理 致病性 

分 类 号：S947.9[农业科学—水产养殖]