中国明对虾卵黄蛋白原基因启动子克隆与分析  

Cloning and analysis of the promoter of Fenneropenaeus chinensis vitellogenin gene

作　　者：谢松[1] 李理想[1] 陈宏健[1] 孙玲玲[1] 柳峰松[1] 

机构地区：[1]河北大学生命科学学院,河北省无脊椎动物系统学与应用实验室,河北保定071002

出　　处：《河北大学学报（自然科学版）》2013年第2期175-180,共6页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目(C2010000256;C2011201027);教育部高等学校博士学科点专项科研基金资助项目(20101301120005);河北大学自然科学基金资助项目(2011218)

摘　　要：为了探明中国明对虾卵黄蛋白原基因启动子表达调控机制,利用DNA步移法克隆了中国明对虾卵黄蛋白原基因启动子及其上游调控序列,总长1 100bp.分析表明,在基因转录起始位点上游-30～-24bp处有1个TATA box,未发现有CAAT box和GC box.同时,在上游调控区还存在有多个可能影响启动子转录活性的顺式作用元件,如NF-κB,YY1和SP1等转录因子结合位点.这些结果为深入研究中国明对虾卵黄蛋白积累及卵子发生过程奠定了基础.In order to investigate the mechanism of the expression and regulation of the Fenneropenae- us chinensis vitellogenin gene, the promoter and the up-stream regulatory fragment of 1 100 bp were ampli- fied by Genome Walker method. Analysis showed that the ＂TATA box＂ was located at -30 --24 bp in the front of the transcription initiation site, but the ＂CAAT box＂ and ＂GC box＂ were absent. Meanwhile, among the upstream regulatory region, there were a few cis-acting elements which might affect the activity of promoter, such as NF-κB, YY1, SP1, and so on. These results laid the foundation for us to study the accumulation of the vitellin and the oogenesis of Fenneropenaeus chinensis in depth.

关 键 词：卵黄蛋白原 DNA步移 启动子 顺式作用元件 

分 类 号：Q81[生物学—生物工程]