超声萃取-柱色谱法分离鉴定乌桕叶抑螺活性成分  

Separation and determination of molluscicidal composition from Sapium sebiferum by supersonic extraction-column chromatography

作　　者：罗坤水[1] 余能富[1] 邹峥嵘[2] 徐林初[1] 

机构地区：[1]江西省林业科学院,南昌330032 [2]江西师范大学生命科学学院

出　　处：《林业科技开发》2013年第2期74-76,共3页China Forestry Science and Technology

基　　金：国家科技支撑项目"林业血防生态安全体系构建技术研究与示范"(2011BAD38B07);国家林业局建设项目"江西省鄱阳湖抑螺防病林试验示范区建设"(林计批字[2007]527号)

摘　　要：根据笔者前期研究结果,乌桕叶乙醇提取物的乙酸乙酯萃取部位具有较强的抑螺活性。为分离鉴定乌桕叶中的抑螺活性物质,采用超声波辅助溶剂提取、溶剂分步萃取和多种柱层析联用等方法从乌桕叶乙醇提取物中分离得到多个单体化合物,依据化合物的理化性质和波谱学特征,鉴定出3个黄酮类化合物,分别为山柰酚、槲皮素和异槲皮苷,均为首次从该植物中得到。Based on previous research, ethyl acetate extraction of ethanol extract of Sapium sebiferum had strong molluscicidal activity. In order to separate and determine molluscicidal composition from Sapium sebiferum leaves, supersonic extraction, fractional solvent extraction and column chromatography were used to separate many constituent. According to their physicochemical properties and spectroscopic characteristics, three flavonoids were seperated, and were identified as kaempferol, quercetin, isoquercitrin. Three compounds were all separated from this plant.

关 键 词：乌桕 抑螺成分 超声萃取 柱色谱法 黄酮 

分 类 号：S858.31[农业科学—临床兽医学]