不同培养液对鹅胚胎体外培养的影响  

Effects of Different Mediums on Goose Embryos Culture in vitro

作　　者：李玉芳[1,2] 庄益芬[1] 张文昌[1] 

机构地区：[1]福建农林大学动物科学学院,福州350002 [2]黄淮学院,河南驻马店463000

出　　处：《中国农学通报》2013年第8期18-20,共3页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划项目"动物快繁关键技术研究与示范"(2011BAD19B04);福建省自然科学基金项目"以鹌鹑干细胞为载体制作转人血清白蛋白基因鹌鹑的研究"(2007J0042)

摘　　要：为了探讨不同培养液对鹅胚胎体外培养的影响,本试验分别用鸡、鸭、鹅3种稀蛋白作培养液对90只鹅胚进行体外培养。结果表明:用鹅稀蛋白作培养液优于鸡稀蛋白和鸭稀蛋白。鹅稀蛋白组在第6、15天时鹅胚存活率显著高于鸭稀蛋白组(P<0.05),鹅胚存活率在第6、15天分别为33.33%、20.00%;在第31天时鹅稀蛋白组鹅胚存活率为3.33%,其他两组为0。综上所述,鹅胚体外培养效果因培养液的不同而不同,在鸡、鸭和鹅稀蛋白3种培养液中,以选用鹅稀蛋白为宜。The studies were conducted to investigate the effect of different medium on goose embryo in vitro,90 goose embryos from natural eggs were cultured in 3 different mediums.The results showed that：the dilute Protein of Goose Eggs （GP） used as culture medium was better than that of chicken （CP） and duck （DP）.Survival rate of goose embryo in GP was significantly higher than that in DP at 6th and 15th days （P0.05）,which were 33.33% and 20% respectively.Survival rate of goose embryo in GP was 3.33% at 31 th days,while the survival rate was 0 in other 2 mediums.All the analysis showed that the effect of different medium on goose embryo in vitro because of different culture medium,which in 3 different mediums,GP was suitable.

关 键 词：鹅 胚胎 稀蛋白 体外培养 存活率 

分 类 号：S835[农业科学—畜牧学]