2种微生物菌剂对堆肥过程中酶变化的影响研究  

Impacts of Endogenous and Exogenous Microbial Inocula on Changes of Enzyme Activities and Relationship Between Enzyme Activities and Composting Process

作　　者：徐智[1] 李季[2] 

机构地区：[1]云南农业大学资源与环境学院,昆明650201 [2]中国农业大学资源与环境学院,北京100193

出　　处：《中国农学通报》2013年第8期175-179,共5页Chinese Agricultural Science Bulletin

基　　金："十二五"国家科技支撑计划"农业废弃物高效循环利用关键技术研究"(2012BAD14B01);公益性行业(农业)科研专项"利用有机(类)肥料调控我国土壤微生物区系关键技术研究"(201103004-3)

摘　　要：为了探讨不同来源的微生物菌剂对堆肥过程的影响机理,进一步为堆肥微生物接种剂的筛选方向和应用提供理论支持,以一种内源微生物M37和一种外源微生物VT作为堆肥接种剂,通过研究和比较这2种不同来源(内源和外源)微生物菌剂对堆肥过程中温度、氧气浓度以及酶(蛋白酶、脲酶、脱氢酶和纤维素酶)活性的动态变化的影响及堆肥过程中温度、氧气浓度变化与酶活性的关系。结果表明,堆肥升温期的温度和氧气浓度变化呈极显著负相关(P<0.01)。内源微生物菌剂有利于堆肥过程快速升温和升温过程中蛋白酶、脲酶的累积;外源微生物菌剂有利于堆肥高温期保持更高的温度和高温期脱氢酶、纤维素酶活性的增强。蛋白酶活性、脲酶活性和温度呈极显著的正相关(P<0.01)。脱氢酶的活性和温度都呈极显著的正相关关系(P<0.01),纤维素酶的活性在高温期和温度呈显著的正相关关系(P<0.05)。In order to investigate the action mechanism of the different sources microbial inocula during the composting process,and further provide theoretical support for the screening and application of compost microbial inoculant.The endogenous inocula （M 37） and exogenous inocula （VT） were as compost Inoculants,studied the effects of indigenous and exogenous inoculation on changes of temperature,oxygen concentration,as well as protease activity,urease activity,dehydrogenase activity and cellulase activity during composting stages in a composting bioreactor.The results showed that,at the beginning of composting,endogenous inocula were beneficial to rapid rising temperature and the cumulation of protease activities and urease activities.At the high-temperature period,exogenous inocula were beneficial to maintain a higher temperature and enhanced dehydrogenase activities and cellulase activities.At the warming and high-temperature period,there was very significant negative correlation between temperature and oxygen concentration.During the period of raising temperature,the protease activity,urease activity and temperature were highly significant positive correlation （P〈0.01）.At the period of raising temperature and high-temperature,dehydrogenase activity and temperature were all highly significant positive correlation between （P〈0.01）.There was a significant positive correlation between the temperature and cellulase activity at the high-temperature period （P〈0.01）.

关 键 词：堆肥 接种剂 酶活性 堆肥反应器 

分 类 号：X705[环境科学与工程—环境工程]