不同地区来源桉树青枯病原菌落生长及幼枝水培接种致病力的差异  

Colonial Growth and Virulence through Water Culture Inoculation of Juvenile Shoots with Eucalypt Bacterial Wilt Pathogens from Distinct Origins

作　　者：罗忠霞[1,2] 魏润鹏[1,3] 房伯平[2] 

机构地区：[1]华南农业大学林学院,广州510642 [2]广东省农业科学院作物研究所,广州510640 [3]嘉汉林业国际有限公司

出　　处：《广西林业科学》2013年第1期1-7,13,共8页Guangxi Forestry Science

摘　　要：不同地区来源的12个青枯病菌株菌落生长特征及桉树幼枝水培接种致病力差异显著,菌落形态存在一定的差异,但是菌株菌落生长与致病值之间没有明显的关联。菌株菌液浓度对致病值影响显著,随浓度提高而加强;用于试验的4个桉树无性系感病性统计上没有显著性差别。菌落生长主要集中在平板划线培养后的4天内;第4天时,平均大小从3.53～27.68 mm。虽然菌株间致病值差异显著,如剔除表现最强及最弱的2个菌株,其余菌株间致病值则没有差别,说明不同地区幼林林木在等同气候环境条件下,受青枯病危害的机率类似,但是,个别立地林分可能因特定病原菌株而受害严重。It was found that 12 Ralstonia solanacearum isolates from distinct origins were obviously differ- ent in colonial growth and virulence through water culture inoculation of eucalypt juvenile shoots, but the colonial growth and virulence did not exhibit relationship. The virulence significantly increased with the concentration of pathogen solution. However, 4 eucalypt clones tested did not show marked difference in susceptibility to the bacterial wilt pathogen. Most of the colony growth on plate occurred in the first 4 days after inoculation, with the average diameter ranging from 3.53 to 27.68 mm on the 4th day. Further a- nalysis showed that if two pathogen isolates with the strongest and weakest virulence respectively were ex- cluded, the remaining isolates with different origins became similar in virulence. This implied that under the same or similar environmental and climatic conditions, most of young eucalypt stands in distant re- gions or places have the likeness in incurring bacterial wilt, while the rest may severely be damaged by certain isolates with strong virulence.

关 键 词：桉树 青枯病 菌株 水培接种 致病力 

分 类 号：S792.39[农业科学—林木遗传育种] S763.11[农业科学—林学]