高效液相色谱法测定白芷维生素E乳膏中维生素E的含量  

Determlnation of vitamin E in compound angelicae dahuricae radix cream by HPLC

作　　者：何荣芬[1] 

机构地区：[1]安徽省淮北市人民医院,安徽淮北235000

出　　处：《安徽医药》2013年第3期399-400,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立高效液相色谱法测定白芷维生素E乳膏中维生素E的含量。方法 Waters e2695 HPLC色谱系统;EmpowerWaters工作站;Waters 2489 UV检测器;Platisil ODS(150 mm×4.6 mm,5μm)色谱柱;流速:1.0 mL.min-1;流动相:甲醇;检测波长284 nm,进样量为20μL。结果维生素E在82.76～1 655.2 mg.L-1的范围内呈良好的线性关系(r=0.999 9)。维生素E的平均加样回收率为100.5%(n=9),RSD=0.87%。结论该方法操作简便、准确、稳定、灵敏度高,适用于白芷维生素E乳膏中维生素E的质量控制。Objective To establish an HPLC method for determination of vitamin E in compound angelicae dahuricae radix cream.Methods Waters e2695 HPLC included empower waters software,waters 2489 UV detector.And platisil ODS column（150 mm×4.6 mm,5 μm）was used.Mobile phase was methanol,the flow rate was 1.0 mL·min-1 and the detection wavelength was 284 nm.The column temperature was set at room temperature.Injection volume was 20 μL.Results The linear range of vitamin E was 82.76~1 655.2 mg·L-1（r=0.999 9）.The average recovery was 100.5%（n=9）,and RSD was 0.87%.Conclusion This method was simple,sensitive,and accurate for the quality control of compound angelicae dahuricae radix cream.

关 键 词：白芷维生素E乳膏 维生素E 高效液相色谱法 

分 类 号：R927[医药卫生—药学]