2,4-D对小鼠精子毒性作用的研究  

Toxic Effect of 2,4-D on Sperms in Mice

作　　者：苏芳菲[1] 胡梦青[1] 梅天资[1] 杨蓓[2] 杨树龙[2] 邹惟莹[2] 邹挺[2] 张大雷[2] 

机构地区：[1]南昌大学第一临床医学院,2009级南昌330006 [2]南昌大学基础医学院生理教研室,南昌330006

出　　处：《南昌大学学报（医学版）》2013年第1期13-15,37,共4页Journal of Nanchang University：Medical Sciences

基　　金：国家自然科学基金(81060056);江西省教育厅资助项目(GJJ12083)

摘　　要：目的研究2,4-二氯苯氧乙酸(2,4-D)对小鼠精子的毒性作用。方法将24只小鼠按随机数字表法分为4组:对照组、2,4-D 50mg·kg-1组(染毒1组)、2,4-D 100mg·kg-1组(染毒2组)、2,4-D 200mg·kg-1组(染毒3组),每组6只。动物经灌胃染毒,1次.d-1。14d后,处死小鼠,计算睾丸指数、附睾指数,并观察精子数量及精子活力、精子活率的情况。结果与对照组、染毒1组比较,染毒2组、染毒3组显著抑制了精子生成,并呈剂量依赖的方式降低了小鼠精子数量(P<0.05);与对照组比较,染毒2组、染毒3组明显降低了小鼠的精子活力(P<0.05),染毒2组、染毒3组呈剂量依赖的方式降低了小鼠的精子活率(P<0.05),染毒1组对小鼠精子活力、精子活率无显著的影响(P>0.05)。结论 2,4-D能够降低小鼠精子质量,从而引起生殖毒性损伤。Objective To investigate the toxic effect of 2,4-dichlorophenoxyacetic acid （2,4-D） on sperms in mice. Methods Twenty-four male mice were randomly divided into four groups：control group,2,4-D 50 mg·kg^-1 treatment group （group 1）, 2,4-D 100 mg·kg^-1 treatment group （group 2） and 2,4-D 200 mg·kg^-1 treatment group （group 3）,with 6 mice in each group.Mice were given intragastric administration of 2,4-D once per day. After 2,4-D exposure for 14 days, mice were sacrificed and testes and epididymis were collected to calculate testicle and epidid-ymis indexes. In addition,the number, activity and motility of sperms were determined. Results Compared with control group, the number,activity and motility of sperms significantly decreasedin group 2 and group 3 （P〈0.05）. However,the low dose of 2,4-D （50 mg·kg^-1） had no toxic effect on mouse sperms （P〉0.05）. Conelusion The 2,4-D exposure can induce reproductive tox-icity through reducing sperm quality.

关 键 词：2  4-二氯苯氧乙酸 毒性 精子数量 精子活力 精子活率 动物 实验 小鼠 

分 类 号：R9[医药卫生—药学] R339.2