甲基橙皮苷和人血清白蛋白相互作用的光谱研究  

Interaction between methyl hesperidin and human serum albumin studied by spectrometric methods

作　　者：李金华[1] 王素敏[1] 朱金花[2] 

机构地区：[1]西安工业大学材料科学与化工学院,陕西西安710032 [2]河南大学化学化工学院,河南开封475004

出　　处：《化学研究》2013年第2期176-179,共4页Chemical Research

基　　金：陕西省教育厅专项科研计划项目(2010JK609)

摘　　要：利用荧光光谱法和红外光谱法研究了甲基橙皮苷(MH)与人血清白蛋白(HSA)的相互作用.结果表明,MH对HSA的荧光有较强的猝灭作用.在296、303、310K温度下,MH与HSA相互作用的结合常数分别为1.77×104,2.65×104,3.53×104 L·mol-1.热力学分析结果表明,MH与HSA之间的结合过程是吸热的并且是自发的;作用力以疏水作用为主,并伴随氢键作用.The interaction between methyl hesperidin （MH） and human serum albumin （HSA） under simulative physiological conditions was studied by spectroscopic methods including fluo- rescence quenching technology and Fourier transform infrared （FT-IR） spectrometry. Results reveal that MH can well quench the intrinsic fluorescence of HSA. The binding constants at 296, 303, and 310 K are 1.77×104 , 2.65×104 , and 3.53×104 L .mol-1 , respectively. Ther- modynamic analyses show that the binding process between MH and HSA is endothermic and spontaneous, and the interaction is dominated by hydrophobic interaction, accompanied by hy- drogen bonding.

关 键 词：甲基橙皮苷 人血清白蛋白 相互作用 荧光光谱 红外光谱 

分 类 号：O657.3[理学—分析化学]