桉树Genomic-SSR和EST-SSR引物的快速筛选与通用性研究  

Rapid Screening and Transferability Analysis of Genomic-SSR and EST-SSR Primers in Eucalypt

作　　者：刘果[1] 张党权[2] 谢耀坚[1] 谷振军[2] 章怀云[2] 

机构地区：[1]国家林业局桉树研究开发中心,湛江524022 [2]中南林业科技大学林业生物技术湖南省重点实验室经济林育种与栽培国家林业局重点实验室经济林培育与保护省部共建教育部重点实验室,长沙410004

出　　处：《林业科学》2013年第2期127-133,共7页Scientia Silvae Sinicae

基　　金：林业公益性行业科研专项(201104003);863计划课题(2011AA100202);湖南省科技厅重点项目(2010NK2008,2011NK2019);广东省林业创新专项(2011KJCX019)

摘　　要：桉树是桃金娘科（Myrtaceae）桉属（Eucalyptus）、杯果木属（Angophora）和伞房属（Corymbia）3个属的树种总称,约有945种。桉树具有生长迅速、轮伐期短、耐干旱、耐贫瘠、适应性广等优良特性,且用途广、经济效益高,因此,桉树现已被世界近百个国家与地区引种,遍布于地中海地区、东南亚、美洲和非洲,目前已经成为世界第二大类造林树种（陈少雄等,2008;欧阳乐军等,2008;祈述雄,2006）。Based on the relevant properties of EST-SSR gene function, and the high polymorphism and potential regulatory function of Genomic-SSR, the polymorphism effect and transferability of EST-SSR and genomic-SSR of eucalypt trees was comparatively studied in this article. A genomic DNA pooling method was developed to rapidly screen eucalypt SSR primers. A total of 340 pairs of eucalypt SSR primers were screened from 395 pairs designed by optimizing SSR-PCR system, and the screening ratio was 86.06%. Genomic-SSR and EST-SSR screened 204 pairs （ratio 83.26%） and 136 pairs （ratio 90.67%）, respectively. The result indicates that these obtained SSR primers have good polymorphism and transferability in eucalypt trees, and are suitable for SSR marker analysis of various eucalypt species. Compared with the conventional single-template screening method, the first round of screening primers by genomic DNA pooling technology has reduced more than 82% of workload and reagent consumption. The findings lay a foundation for all-aspect, fast and accurate application of SSR marker technology in eucalypt trees.

关 键 词：SSR标记 桉树 引物筛选 基因组DNA混合池 

分 类 号：S718.46[农业科学—林学] Q943.2[生物学—植物学]