蛋白溶液浓度对动态光散射测量结果的影响  

The Influence of Protein Concentration on the Results of Dynamic Light Scattering Measurement

作　　者：高潮[1] 张钰羚[1] 郭永彩[1] 

机构地区：[1]重庆大学光电工程学院光电技术及系统教育部重点实验室,重庆400030

出　　处：《光散射学报》2013年第1期54-58,共5页The Journal of Light Scattering

基　　金：教育部博士点基金项目(20090191110026)

摘　　要：蛋白质在生命活动中起着重要作用,一直是人们研究的热点,动态光散射技术由于其快速、准确、对样品无损坏等优点,而成为研究蛋白质的重要手段之一。本文采用美国布鲁克海文公司提供的BI-200SM激光散射仪对浓度分别为0.1mg/mL、0.5mg/mL、1mg/mL、5mg/mL、10mg/mL的牛血清白蛋白溶液进行动态光散射测量,讨论了光强自相关曲线和粒径测量的相对误差随浓度的变化关系,研究了蛋白浓度对测量结果的影响。实验结果表明,在一定浓度范围内,光强自相关函数曲线的稳定性随浓度增加而增加,而自相关曲线的迟豫时间随着溶液浓度的增加而减小,粒径测量的相对误差也随着浓度的增加而逐渐减小。Protein plays an important role in the life activities. So it has been a focus for re searchers. Due to its advantages of rapid, accurate, no sample damage and so on, the e merging dynamic light scattering technique becomes an important tool to research protein. BI 200SM laser light scattering instrument of U. S. Brookhaven is used to measure bo vine serum albumin solution in different concentrations（0.1 mg/mL, 0.5 mg/mL, 1 rng/ mL, 5 mg/mL and 10 mg/mL）; to research the relationship between the autocorrelation function curve of photons intensity or the relative error of measuring particle size and con centration. The results show that, within a certain concentration range, the autocorrela tion function curve of photons intensity tends to stable smooth with increasing concentra tion, while the delay time decreases and the relative error of measuring particle size decrea ses.

关 键 词：动态光散射 牛血清白蛋白 自相关函数曲线 弛豫时间 稳定性 

分 类 号：O436.2[机械工程—光学工程]