猪骨桥蛋白基因的组织差异表达  

Differential expression in tissues of porcine OPN gene

作　　者：李仕新[1] 李加琪[2] 田允波[1] 陈赞谋[2] 古飞霞[1] 唐军[1] 

机构地区：[1]仲恺农业工程学院生命科学学院,广东广州510225 [2]华南农业大学动物科学学院广东省农业动物基因组学与分子育种重点实验室,广东广州510642

出　　处：《仲恺农业工程学院学报》2013年第1期5-8,共4页Journal of Zhongkai University of Agriculture and Engineering

基　　金：国家生猪现代产业技术体系建设专项(CARS-36);广东省自然科学基金(10451022501005709);广东省科技计划(B1101328)资助项目

摘　　要：为了研究猪骨桥蛋白基因(Osteopontin,OPN)组织特异性的表达规律,采用实时荧光定量PCR(Real-time quantitative PCR,qRT-PCR)分析方法,以β-actin基因为内参,对猪OPN基因在心、肝、脾、肺、肾、大脑、小脑、垂体、下丘脑和子宫内膜等10种组织中的mRNA表达水平进行了检测.结果表明,OPN基因在子宫内膜的表达量为最高,其次是在小脑,在心、肝、脾和肺也有不同程度的表达,而在肾、下丘脑和垂体的表达量很少,从而提示OPN基因在子宫内膜中可发挥重要作用.In order to study the tissue-specific expression pattern of porcine OPN gene, mRNA expression levels in ten tissues of Landrace ~ Yorkshire pigs were detected by using real-time quantitative PCR（qRT-PCR）, and these tissues were heart, liver, spleen, lung, kidney, cerebrum, cerebellum, pituitary, hypothalamus and endometrium. The results showed that the levels of OPN gene expression in endometrium were the highest and that there were different expression levels in cerebellum, heart, liver, spleen, lung, and rarely in the kidney, hypothalamus and pituitary. The tissue-specific expression pattern of porcine OPN gene suggests that OPN may play an important role in the endometrium.

关 键 词：猪 OPN基因 组织特异性表达 实时荧光定量PCR MRNA 

分 类 号：S813.2[农业科学—畜牧学]