色氨酸在自制牛血清白蛋白手性柱上的拆分  

Enantioseparation of Tryptophan on a Homemade Bovine Serum Albumin Based Chiral Stationary Phase

作　　者：亢珊珊[1] 黄洁云[1] 邓翠瑶[1] 叶丽虾[1] 姚碧霞[1] 翁文[1] 

机构地区：[1]漳州师范学院化学与环境科学系,福建漳州363000

出　　处：《福建分析测试》2013年第2期5-9,共5页Fujian Analysis & Testing

基　　金：国家自然科学基金(20705031);福建省自然科学基金杰出青年基金项目(2012J06005);福建省高等学校新世纪优秀人才支持计划(JK2011030)资助

摘　　要：利用三氯聚氰活化的氨丙基硅胶与牛血清白蛋白反应,快速而经济地制得牛血清白蛋白手性固定相。在反相模式下,将该手性固定相用于色氨酸的拆分,系统探讨了流动相pH值、柱温、有机修饰剂的种类及含量等对手性拆分的影响。色氨酸在自制牛血清白蛋白手性柱上得到了理想的拆分,分离因子可达4.33。A bovine serum albumin （BSA） based chiral stationary phase was prepared conveniently from aminopropyl silica gel with 2, 4, 6-trichloro-1, 3, 5-triazine as an activator. Enantioseparation of tryptophan was performed on this chiral stationary phase under the reverse-phase mode. The effects of pH value of mobile phase, type and content of organic modifier, and column temperature on the enantioseparation of tryptophan were investigated. The solute achieved favorable enantioseparation, and the separation factor could reach 4.33.

关 键 词：手性固定相 手性拆分 牛血清白蛋白 色氨酸 三氯聚氰 

分 类 号：O657.7[理学—分析化学]