用RAPD技术检测龙须菜色素突变体基因组的变化  

USING RAPD TECHNIQUE TO DETECT GENOMIC CHANGE OF PIGMENT MUTANTS OF GRACILARIA LEMANEIFORMIS

作　　者：李向峰[1] 隋正红[1] 张学成[1] 

机构地区：[1]青岛海洋大学海洋生命学院,青岛266003

出　　处：《海洋与湖沼》2000年第4期392-397,共6页Oceanologia Et Limnologia Sinica

基　　金：国家攀登计划B资助项目!PDB 6-4 -3号;曾呈奎海洋科学发展基金资助项目!( 1 996)

摘　　要：龙须菜野生型于 1 996年采自青岛太平角 ,经性别、相态确认后进行实验 ;突变株为野生型经诱变的配子体。用RAPD技术检测野生型龙须菜及 3株色素突变体的基因组特性及差异。共选用 1 0个随机引物进行扩增 ,产生 1 67条DNA片段 ,检测到 38个多态位点 ,并显示gr1 84在 0 .66、0 .79、2 .0kb处的特征带。结果表明 ,野生型株藻与突变株基因组有明显差异 ,在 3个突变藻株中 ,有 2株绿色突变体的基因组更相近。RAPD was used to disclose genomic characters and difference of isolates of wild type and three pigment mutants of Gracilaria lemaneiformis . The wild type strains were collected from Taipingjiao cape of Qingdao and experimented after sex and phase identification. A total of 10 random primers were screened for DNA amplification. 167 bands and 38 polymorphic DNA loci were detected, most of which had the molecule weight from 0.32kb to 4.0kb. The result show predominant difference between the genome of wild type strain and mutants, though they belonged to the same species. The polymorphic pattern of the three mutants produced by primer P-3 and P-6 were similar and obviously different from that of the wild type strain, which indicated that partial mutation of the three mutants were not far from each other. The sites and mutation character of the mutants were similar, and the biochemical difference between the wild type strain and the mutants lay in this. The genome of the two green mutants was closer in relationship among the three mutants. Characteristic bands of gr184 with molecular weight of 0.66kb, 0.79kb and 2.0kb were disclosed, which can be used as specific markers of gr184 in the following studies.

关 键 词：龙须菜 色素突变体 RAPD 基因组 

分 类 号：S968.439[农业科学—水产养殖]