UPLC法测定阳和平喘颗粒中芥子碱硫氰酸盐的含量  

Determination of sinapine thiocyanate in YangHePingChuan granules by UPLC

作　　者：桂洁[1,2] 汪永忠[2] 夏伦祝[2] 高家荣[2] 韩燕全[2] 陈曦[1,2] 

机构地区：[1]安徽中医学院,合肥230031 [2]安徽中医学院第一附属医院国家中医药管理局中药制剂三级实验室,合肥230031

出　　处：《海峡药学》2013年第3期42-43,共2页Strait Pharmaceutical Journal

基　　金：2011年安徽省中医发展省级专项补助资金项目(201138)

摘　　要：目的建立阳和平喘颗粒中芥子碱硫氰酸盐的含量测定方法。方法采用超高效液相色谱法,以Acquity UPLC BEH C18柱(100mm×2.1mm,1.7μm)为色谱柱,乙腈-0.08moL.L-1磷酸二氢钾溶液(13∶87)为流动相;流速:0.25mL.min-1;柱温:30℃;检测波长326nm。结果芥子碱硫氰酸盐在0.105～1.050μg范围内呈良好的线性关系,平均回收率为:99.55%,RSD=1.82%。结论本法准确可靠,简单易行,可用于该制剂的质量控制。OBJECTIVE To establish a method for determination of sinapine thiocyanate in YangHePingChuan granules by UPLC. METHODS UPLC method was adopted. The determination was performed on the Acquity UPLC BEH C18（ 100mm × 2. lmm, 1.7μm） column with mobile phase consisted of acetonitrile-0. 08 mol · L-1 potassium dihydrogen phosphate（13：87） at the flow rate of 0. 25mL · min-1. The detection wavelength was set at 326nm and the column temperature was 30℃. RESULTS The linear ranger of sinapine thiocyanate was 0. 105 - 1. 05μg with an average recovery of 99.55% （ RSD = 1.82%, n = 6）. CONCLUSION The method is accurate, reliable, simple and practical, it can be used for the quality control of granules.

关 键 词：阳和平喘颗粒 UPLC 芥子碱硫氰酸盐 含量测定 

分 类 号：R927.2[医药卫生—药学]