TNF-α增强膀胱上皮细胞清除细胞内肺炎克雷伯杆菌  

Clearance of intracellular Klebsiella pneumoniae by human urinary epithelial cells enhanced by TNF-α

作　　者：张虎山[1] 路会侠[1] 熊文碧[2] 刘伟[1] 吴琦[1] 

机构地区：[1]四川大学华西基础医学与法医学院感染免疫研究室,四川成都610041 [2]四川大学华西基础医学与法医学院药理教研室,四川成都610041

出　　处：《四川生理科学杂志》2013年第1期1-4,共4页Sichuan Journal of Physiological Sciences

基　　金：国家自然科学基金(编号:30270688;30570790)

摘　　要：目的:研究TNF-α对膀胱上皮细胞内肺炎克雷伯杆菌生存的影响。方法:以肺炎克雷伯杆菌侵入体外培养的人膀胱上皮细胞(T24细胞)为模型,观察在TNF-α等细胞因子处理条件下,不同时间点细胞内细菌数量变化。结果:单独使用TNF-α使T24细胞内的肺炎克雷伯杆菌K5株细菌数量明显减少,联合使用TNF-α与IFN-γ使胞内活菌数量更显著的减少。而IL-1β对细胞内活菌数量无明显影响。过氧化氢酶可以有效抑制TNF-α与IFN-γ刺激的T24细胞抗菌作用,而一氧化氮合酶抑制剂L-NAME无抑制作用。结论:TNF-α能够增强膀胱上皮细胞对抗细胞内肺炎克雷伯杆菌,抗菌机制与细胞产生活性氧(ROS)有关。Objective： To investigate the effect of proinflammation cytokine TNF-α on the antibacterial activity of urinary epithelial cells against intracellular Klebsiella pneurnoniae. Methods： Monolayers of T24 cells were grown to confluence in 24-well plate. Cells were infected with Klebsiella pneumoniae strain K5, a clinical isolate（approximately 100 bacteria per epithelial cell） for 2 hours. Extracellular bacteria were killed by gentamicin and the cells were incubated with proinflammation cytokines （TNF-α, IFN-γ, IL-1β）. After 24 and 48 hours from the initial infection, the cells were lysed with Triton X-100 and viable intracellular bacteria were quantified by plating appropriate dilutions on LB agar plates. Results： In comparison with control, the number of viable intracellular Klebsiella pneumoniae decreased significantly in the cells treated with TNF-α. Combination of TNF-α and IFN-γ further decreased the number of viable intracellular bacteria. On the other hand, IL-1β had no significant effect on the number of viable intracellular Klebsiella pneurrumiae. Antibacterial activity induced by TNF-α and IFN-γ was significantly inhibited by catalase. Conclusion： The clearance of intracellular Klebsiella pneumoniae by human urinary epithelial cells could be enhanced by TNF-α and reactive oxygen species（ROS） were involved in the antibacterial activity.

关 键 词：膀胱上皮细胞 TNF-Α 肺炎克雷伯杆菌 

分 类 号：R378[医药卫生—病原生物学]