盐度对牟氏角毛藻生长及总脂含量的影响  

Effects of Varying Salinity on Growth and Total Lipid Content of Chaetoceros muelleri

作　　者：班剑娇[1] 雷静静[1] 王志强[1] 张旭芳[1] 冯佳[1] 谢树莲[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《安徽农业科学》2013年第6期2621-2622,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金资助项目(31170193)

摘　　要：[目的]研究不同盐度对牟氏角毛藻(Chaetoceros muelleri)生长及总脂含量的影响,以确定最高油脂产率的盐度。[方法]采用微挑法进行藻株的分离纯化;采用血球计数法进行细胞计数、干重法测定藻株生物量;采用氯仿-甲醇法进行藻株总脂含量的测定。[结果]盐度为20‰时,牟氏角毛藻生长最快,最大细胞密度为2.1×106个/ml,干重为58.89 mg/L,;盐度为25‰时,牟氏角毛藻总脂含量最高,达到29.93%。[结论]综合评价其生长及总脂积累,得出盐度为20‰时,牟氏角毛藻总脂产率最高,但与盐度25‰时差异不大。[Objective] The effects of three different salinity on growth and total lipid content of Chaetoceros muelleri were researched to obtain the salinity under the highest yield of oil.[Method] C.muelleri was isolated and purified by micro-picking method;blood count method was used for determining cell density and dry weight method was used for determining biomass;total lipid content was measured by chloroform-methanol extraction method.[Result] The results showed that the highest cell density（2.1×106 ind/ml） and dry weight（58.89 mg/L） of C.muelleri were obtained at salinity of 20‰.The highest total lipid content（29.93%） was obtained at salinity of 25‰.[Conclusion] Under salinity of 20‰,the highest lipid productivity was obtained.There is no significant difference between salinity of 20‰ and 25‰.

关 键 词：牟氏角毛藻 盐度 生物量 总脂含量 

分 类 号：S181.3[农业科学—农业基础科学]