水榆花楸微型繁殖技术的研究  

Study on Micro Propagation Technology of Sorbus alnifolia

作　　者：宋洪文[1] 张象君[1] 辛宝英[1] 王小岚[1] 

机构地区：[1]黑龙江林业职业技术学院生态工程系,黑龙江牡丹江157011

出　　处：《安徽农业科学》2013年第5期1898-1899,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究水榆花楸的微型繁殖技术。[方法]以带侧芽的水榆花楸茎段为外植体,研究不同外植体消毒时间、基本培养基及植物生长调节剂种类和浓度对水榆花楸侧芽诱导、丛生芽增殖及生根的影响。[结果]外植体的最佳消毒时间为7 min;侧芽的最佳诱导培养基为MS+KT 2.0 mg/L+IBA 0.2 mg/L;丛生芽的最佳增殖培养基为MS+KT 2.5 mg/L+IBA 0.1 mg/L;最佳生根培养基为1/2MS+IBA 0.5 mg/L。[结论]该研究为水榆花楸苗木的大规模工业化生产提供了技术支持。[Objective] The aim was to study the micro propagation technology of Sorbus alnifolia.[Method] The effects of different sterilization time,basic culture mediums,plant growth regulator types and concentrations on the lateral buds induction,clustered buds proliferation and rooting of S.alnifolia were studied by using stem segments with lateral buds as explants.[Result] The best sterilization time of explants was 7 min;the best induction medium of lateral buds was MS＋KT 2.0 mg/L＋IBA 0.2 mg/L;the best proliferation medium of clustered buds was MS＋KT 2.5 mg/L＋IBA 0.1 mg/L;the best rooting medium was 1/2MS＋IBA 0.5 mg/L.[Conclusion] This study provided technical support for the large-scale industrialization production of S.alnifolia seedlings.

关 键 词：水榆花楸 微型繁殖 培养基 诱导率 增殖率 生根率 

分 类 号：S792.25[农业科学—林木遗传育种]