转基因产品检测技术研究进展  

Research Progress of Genetically Modified Organisms Detection Technique

作　　者：翟聪聪[1,2] 陈洪俊[2] 李志红[1] 乔文婕[1,2] 颜春凤[3] 付伟[1,2] 

机构地区：[1]中国检验检疫科学研究院,北京100029 [2]中国农业大学农学与生物技术学院,北京100193 [3]贵州师范大学,贵州贵阳550001

出　　处：《安徽农业科学》2013年第5期1900-1901,2097,共3页Journal of Anhui Agricultural Sciences

基　　金：转基因重大专项课题(2011ZX08012-001)

摘　　要：随着转基因产品的种类和数量逐年增加,转基因产品的安全性也越来越受到全球广泛关注,迫切需要建立有效的转基因产品检测技术。文中简要介绍了现有的转基因产品检测技术及方法,目前转基因检测方法主要是基于外源蛋白靶标的检测方法和基于核酸的检测方法,如酶联免疫吸附法、PCR技术、恒温扩增技术、生物传感器及数码PCR等,并着重介绍了个各方法的优缺点及转基因产品检测的困难及趋势。With the increasing of GMO（genetically modified organisms）,the safety of GMO has attracted more and more worldwide attention,it is urgently needed to establish effective GMO detection technology.This review briefly introduced the existing primary GMO detection technology based on detection of protein and nucleic acid,such as ELISA,PCR,isothermal amplification technology,biosensor technology and digital PCR and so on,meanwhile emphasized their advantages,disadvantages and the difficulties,trend of GMO detection.

关 键 词：转基因产品 检测 灵敏度 特异性 

分 类 号：S188[农业科学—农业基础科学]