镉、铜胁迫下拟南芥基因组DNA甲基化的MSAP分析  

Analysis of Genomic DNA Methylation in Arabidopsis thaliana under Cd and Cu stress using Methylation-sensitive Amplification Polymorphism Technique

作　　者：赵晓辉[1] 徐正进[1] 刘宛[2] 王鹤潼[1] 李照令[2] 马珊珊[2] 孙晓霞[3] 

机构地区：[1]沈阳农业大学水稻研究所,辽宁沈阳110866 [2]中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室,辽宁沈阳110016 [3]辽宁大学,辽宁沈阳110036

出　　处：《安徽农业科学》2013年第5期1911-1915,共5页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金面上项目(20977095);辽宁省自然科学基金项目(201202224);国家科技重大专项(2012ZX07505-001);国家基金重点项目(40930739);沈阳大学区域污染环境生态修复教育部重点实验室基金资助

摘　　要：[目的]研究不同浓度Cd、Cu胁迫下拟南芥幼苗基因组DNA的甲基化水平和状态变化。[方法]以0、0.5和5.0 mg/L Cd2+、0.5和5.0 mg/L Cu2+处理21 d的拟南芥为材料,提取基因组DNA,进行甲基化敏感扩增多态性(methylation sensitive amplification polymor-phism,MSAP)分析。[结果]0、0.5 mg/L Cd2+、5.0 mg/L Cd2+、0.5 mg/L Cu2+和5.0 mg/L Cu2+处理组DNA的MSAP比率分别为30.2%、34.7%、41.0%、33.9%、39.2%。0.5 mg/L Cd2+、5.0 mg/L Cd2+、0.5 mg/L Cu2+、5.0 mg/L Cu2+处理组的DNA甲基化多态性比例分别为2.6%、13.2%、2.4%、11.2%。[结论]Cd、Cu胁迫下拟南芥基因组DNA甲基化水平的增加及DNA甲基化多态性的提高与Cd、Cu处理浓度呈显著正相关,且Cd对DNA甲基化水平和多态性改变的作用大于Cu。[Objective] To study the effect of Cd and Cu stress on the genomic DNA methylation levels and states of Arabidopsis thaliana.[Method] Arabidopsis thaliana was planted in different concentration nutrient solution with 0,0.5 mg/L Cd,5.0 mg/L Cd,0.5 mg/L Cu,5.0 mg/L Cu for 21 days.Genomic DNA was extracted to conduct methylation-sensitive amplification polymorphism（MSAP） analysis.[Result] MSAP rate of 0,0.5 mg/L Cd,5.0 mg/L Cd,0.5 mg/L Cu,5.0 mg/L Cu was 30.2%,34.7%,41.0%,33.9%,39.2%,DNA methylation polymorphism rate of 0.5 mg/L Cd,5.0 mg/L Cd,0.5 mg/L Cu,5.0 mg/L Cu were 2.6%,13.2%,2.4%,11.2%,respectively.[Conclusion] Arabidopsis thaliana genome DNA methylation level and methylation polymorphism under Cd and Cu stress increases with the concentration increased obviously,the effect of Cd for the change of DNA methylation level and polymorphism is greater than Cu.

关 键 词：拟南芥 DNA甲基化 镉胁迫 铜胁迫 MSAP 

分 类 号：S188[农业科学—农业基础科学]