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作 者:秦瑜丽
机构地区:[1]山西振东制药股份有限公司,山西长治047100
出 处:《安徽农业科学》2013年第5期2248-2249,2260,共3页Journal of Anhui Agricultural Sciences
摘 要:[目的]建立应用高效液相法测定复合维生素片中叶酸和维生素B12含量的方法。[方法]色谱柱用菲罗门lunaC18(4.6×250 mm,5μm),流动相为甲醇-0.028 mol/L磷酸氢二钠水溶液(用磷酸调pH到3.5)(21∶79),流速1.0 ml/min,检测波长为361 nm,进样量为20μl,柱温为25℃。[结果]维生素B12在6.65~66.50 ng范围内呈现良好的线性关系,回归方程是y=0.495 1x+1.342 8(R2=0.999 4,n=6),平均回收率为96.83%,RSD为0.82%(n=6)。叶酸在80~800 ng范围内呈现良好的线性关系,回归方程是y=0.021 1 x+0.661 1(R2=0.998 8,n=6),平均回收率为95.56%,RSD为1.54%(n=6)。[结论]高效液相色谱法灵敏度高,操作简便,重现性好,结果准确,可以用于复合维生素片中叶酸和维生素B12的含量测定。[Objective] To establish a method for the determination of folic acid and vitamin B12 in compound vitamin pills by HPLC [Method] The samples were separated on Phenomenex luna C18(4.6×250 mm,5 μm),eluted with mixture of methanol-0.028 mol/L Na2HPO4 solution(adjusted to pH 3.5 with phosphoric acid)(21∶79) at 1.0 ml/min.The injection volume was 20 μl and the detector wavelength was set at 361 nm.[Result] Vitamin B12 was good linear over the range 6.65-66.50 ng(y=0.495 1x+1.342 8,R2=0.999 4,n=6).The average recovery rate for vitamin B12 was 96.83%,and RSD was 0.82%(n=6).Folic acid was good linear over the range 80-800 ng(y=0.021 1x+0.661 1,R2=0.998 8,n=6).The average recovery rate for folic acid was 95.56%,and RSD was 1.54%(n=6).[Conclusion] This method was simple,stable,precise and accurate.So this study can be used in determination of folic acid and vitamin B12 in compound vitamin pills.
分 类 号:S121[农业科学—农业基础科学]
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