基于ITS序列分析对紫花地丁的分子鉴别  

Molecular authentication of Viola yedoensis Makino and adulterants by analysis of rDNA ITS sequences

作　　者：朱烨[1] 张春[1] 庄元春[1] 税丕先[1] 

机构地区：[1]泸州医学院药学院,泸州646000

出　　处：《中华中医药杂志》2013年第4期918-920,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81001700)~~

摘　　要：目的:通过研究比较紫花地丁与其易混中药材ITS序列的差异和规律,建立紫花地丁与其易混药材之间的分子鉴别方法。方法:分别从紫花地丁和易混品中提取总DNA,用ITS序列通用引物扩增,扩增产物双向测序。结果:紫花地丁与其他易混品在ITS1和ITS2区段存在特异鉴别位点共8个。结论:根据ITS序列特征,能有效区分紫花地丁和其他易混中药材。ITS序列是紫花地丁鉴定的有效的分子标记。Objective： The rDNA ITS sequences of Viola yedoensis Makino and the adulterants were analyzed to establish the molecular biological method for authentication of Viola yedoensis Makino and the adulterants.Methods： Total DNA of Viola yedoensis Makino and the adulterants were extracted.The ITS sequence was amplified by universal primer.The PCR products were directly sequenced and both directions were sequenced.Results： 8 specific authenticable sites were found in the ITS1 and ITS2 region.Conclusion： The rDNA ITS sequence can be used as good marker for authenticating Viola yedoensis Makino from the adulterants.

关 键 词：紫花地丁 ITS序列分析 中药鉴定 

分 类 号：R282.5[医药卫生—中药学]