复方丹参滴丸治疗颅脑外伤伴发癫痫的实验研究  

Experimental study on the effect of Compound Danshen Dripping Pill on posttraumatic epilepsy in rats

作　　者：蒋莉娅[1] 龚镭[1] 黄继人 戴建良 唐志放 房文峰 

机构地区：[1]无锡市第二人民医院,无锡214002 [2]无锡市中西医结合医院,无锡214041

出　　处：《中华中医药杂志》2013年第4期1117-1121,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81000498)~~

摘　　要：目的:探讨复方丹参滴丸(CDDP)对颅脑外伤后伴发癫痫(PTE)的作用机制。方法:通过制作PTE大鼠模型,不同浓度CDDP治疗21d后,Western Blot检测各组脑海马组织中诱导型一氧化氮合酶(iNOS)、癫痫灶区γ-氨基丁酸B受体亚单位(GABABR1)和谷氨酸受体亚单位(GluR1)、前脑组织中突触后致密物蛋白质95(PSD95)的表达水平。结果:正常组和CDDP高剂量治疗组脑海马组织中iNOS和癫痫灶区GluR1表达水平均低于模型组(P<0.05);两组癫痫灶区GABABR1和前脑组织中PSD95表达水平均高于模型组(P<0.01,P<0.05)。结论:复方丹参滴丸治疗PTE有效,可能与其抑制PTE后脑海马组织内中iNOS和癫痫灶区GluR1的表达,并促进癫痫灶区GABABR1和前脑中PSD95的表达有关。Objective： To study the effective mechanism of Compound Danshen Dripping Pill on posttraumatic epilepsy（PTE）.Methods： After the models of PTE in rats established,the groups were respectively fed with compound Danshen Dripping Pills of different concentrations.Twenty-one days later,expression of iNOS in Hippocampus organization,GluR1 and GABABR1 in epileptic foci area,PSD95 in forebrain of all groups were detected by Western Blot technology.Results： Expression of iNOS in Hippocampus organization and GluR1 in epileptic foci area of normal group and Compound Danshen Dripping Pill high concentration group were lower than model group（P0.05）.And expression of GABABR1 in epileptic foci area and PSD95 in forebrain of these two groups were higher than model group（P0.01,P0.05）.Conclusion： Compound Danshen Dripping Pill has a curative effect on posttraumatic epilepsy,and it may be related to the suppression expression of iNOS in Hippocampus organization and GluR1 in epileptic foci area,and promote expression of GABABR1 in epileptic foci area and PSD95 in forebrain.

关 键 词：复方丹参滴丸 外伤性癫痫 诱导型一氧化氮合酶 γ-氨基丁酸B受体亚单位 谷氨酸受体亚单位 突触后致密物蛋白质95 

分 类 号：R285.5[医药卫生—中药学]