雌激素与机械张应力对骨重建相关因子的作用  

Changes in bone remodeling-related factors under estrogen and tensile stress

作　　者：彭伟[1] 廖春晖 钟小龙[1] 陈珊[1] 

机构地区：[1]中山大学附属第一医院口腔正畸科,广东省广州市510080 [2]广州市妇女儿童医疗中心口腔科,广东省广州市510623

出　　处：《中国组织工程研究》2013年第7期1141-1145,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:牙槽骨是人体骨代谢最活跃的区域之一,对于在正畸治疗过程中的女性患者容易受到雌激素水平的影响。目的:观察雌激素对机械张应力作用下的牙周膜成纤维细胞骨重建相关因子骨保护素、核因子κB受体活化子配体mRNA表达水平的影响。方法:利用一定浓度雌激素干预人牙周膜成纤维细胞后使用四点弯曲加力装置对细胞进行不同时间段的机械张应力加载,并利用RT-PCR检测雌激素与机械张应力共同作用后牙周膜成纤维细胞骨保护素、核因子κB受体活化子配体基因表达变化。结果与结论:雌激素刺激后再进行机械张应力加载的人牙周膜成纤维细胞跟单纯受到机械张应力作用的人牙周膜成纤维细胞相比,骨保护素mRNA表达量明显上调,而核因子κB受体活化子配体mRNA表达量显著下降。实验表明雌激素对机械张应力状态下的牙周膜成纤维细胞骨重建相关因子核因子κB受体活化子配体、骨保护素有较强调控作用。BACKGROUND： Alveolar bone is one of the most active areas in bone metabolism, and it is liable to be affected by estrogen in orthodontic treatment for female patients. OBJECTIVE： To investigate the effect of estrogen on receptor activator of nuclear factor kappa B ligand and osteoprotegerin secreted by human periodontal ligament fibroblasts under mechanical tensile stress METHODS： Human periodontal ligament fibroblasts were subjected to certain tensile stress loaded by four-point bending strain system after stimulated by different concentrations of estrogen. Reversetranscription-PCR detection was used to investigate the changes of bone reconstruction related factors RESULTS AND CONCLUSION： Compared with pure machinery stimulation, human periodontal ligament fibrobtasts intervened by estrogen expressed an osteoprotegerin mRNA raised trend, and a decreased trend in the mRNA expression of receptor activator of nuclear factor kappa B ligand. Estrogen can strongly modulate the mRNA expression of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in human periodonta ligament fibroblasts under mechanical tensile stress.

关 键 词：组织构建 软骨组织构建 牙周膜成纤维细胞 张应力 雌激素 骨保护素 核因子κB受体活化子配体 骨重建 组织构建图片文章 

分 类 号：R318[医药卫生—生物医学工程]