基质细胞衍生因子1α培养心肌细胞和成纤维细胞的增殖与迁移  

Proliferation and migration of cardiomyocytes and cardiac fibroblasts cultured with stromal cell-derived factor-1 alpha

作　　者：侯传举[1] 齐岩梅 张端珍[1] 王琦光[1] 崔春生[1] 匡丽[1] 陈兵[1] 

机构地区：[1]解放军沈阳军区总医院先心病内科,辽宁省沈阳市110840 [2]辽宁省金秋医院心内科,辽宁省沈阳市110016

出　　处：《中国组织工程研究》2013年第7期1220-1227,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金面上项目(30972956)~~

摘　　要：背景:在心脏缺损边缘造成新创面能够促进缺损自愈,基质细胞衍生因子1α能够促进血管生成以及心脏功能。目的:探讨物理损伤及基质细胞衍生因子1α对心肌细胞增殖的影响及心肌细胞对心脏成纤维细胞迁移的趋化作用。方法:原代分离培养大鼠心肌细胞和心脏成纤维细胞,采用刮伤法建立物理损伤模型,并应用10-160μg/L的基质细胞衍生因子1α进行干预;CCK-8法检测心肌细胞的增殖能力;Transwell法检测心肌细胞趋化下心脏成纤维细胞的迁移能力。结果与结论:不同浓度的基质细胞衍生因子1α均可提高物理损伤下心肌细胞的增殖能力,尤以80μg/L基质细胞衍生因子1α作用下心肌细胞增殖最明显。同时,物理损伤联合基质细胞衍生因子1α培养的心肌细胞可显著增加心脏成纤维细胞的迁移能力,并且随着趋化培养时间的延长,迁移能力增加更明显。可见基质细胞衍生因子1α能提高物理损伤心肌细胞的增殖能力,物理损伤联合基质细胞衍生因子1α培养的心肌细胞对心脏成纤维细胞的迁移具有促进作用。BACKGROUND： New wound in the border of defected hearts can promote self-healing, and stromal cell-derived factor-1 alpha can promote angiogenesis and cardiac function. OBJECTIVE： To investigate the effects of mechanical injury and stromal cell-derived factor-1 alpha on the proliferation of cardiomyocytes and the effects of cardiomyocytes on chemotactic migration of cardiac fibroblasts. METHODS： Rat cardiomyocytes and cardiac fibroblasts were cultured primarily. Rat cardiomyocytes were injured mechanically by scratching, and treated with 10-160 pg/L stromal cell-derived factor-1 alpha. The Cell Counting Kit-8 assay was employed to evaluate the proliferation of cardiomyocytes and the Transwell migration assay was used to detect the chemotaxis and migration capability of cardiac fibroblasts. RESULTS AND CONCLUSION： Under mechanical injury condition, stromal cell-derived factor-1 alpha significantly promoted the growth of cardiornyocytes with the maximum effect occurring at 80 pg/L. In addition, mechanically injured and stromal cell-derived factor-1 alpha cultured cardiomyocytes remarkably promoted the migration of cardiac fibroblasts in a time-dependent fashion. These findings indicate that stromal cell-derived factor-1 alpha could promote the growth of cardiomyocytes under mechanical injury condition; otherwise, mechanically injured and stromal cell-derived factor-1 alpha cultured cardiomyocytes could promote the migration of cardiac fibroblasts.

关 键 词：组织构建 组织构建与生物活性因子 心肌细胞 心脏成纤维细胞 基质细胞衍生因子1Α 物理损伤 先天性心脏缺损 房间隔缺损 室间隔缺损 动脉导管未闭 增殖 趋化 国家自然科学基金 组织构建图片文章 

分 类 号：R318[医药卫生—生物医学工程]