20份甜高粱遗传多态性的RAPD和SSR分析  

Genetic Polymorphism of 20 Sweet Sorghum Cultivars as Revealed by RAPD and SSR Markers

作　　者：徐影[1] 詹秋文[1] 董书军[1] 李杰勤[1] 林平[1] 

机构地区：[1]安徽科技学院,安徽凤阳233100

出　　处：《华北农学报》2013年第1期12-18,共7页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31071470);科技部"十二五"农村领域国家科技计划课题(2011BAD17B03);科技部农业科技成果转化资金项目(2008GB2C300125);安徽省"115"产业创新团队(皖人才办[2009]2号)

摘　　要：利用RAPD和SSR分子标记技术,旨在探讨来自不同地区的20份甜高粱杂交种和品种资源遗传多态性及亲缘关系。试验结果表明,筛选出31条RAPD引物在20份甜高粱的DNA中产生稳定清晰条带数为202条,其中多态性片段167条,多态率为82.67%,品种间的遗传相似系数(GS)为0.613～0.870,平均值为0.744;筛选出91对SSR引物在20份品种资源的DNA中扩增片段数为297条,其中多态性片段267条,多态率为89.90%,品种间的遗传相似系数(GS)为0.555～0.837,平均值为0.695;20份甜高粱的2种分子标记的单独聚类分析结果表现出一定差异,但2种趋势大致相近;结合RAPD和SSR这2种标记联合数据的品种聚类分析结果,更能全面客观地反映品种间的遗传多态性及亲缘关系。This study analyzed the genetic polymorphism and relationship of 20 sweet sorghum cultivars by RAPD and SSR molecular markers.The results showed that 31 polymorphism primers were obtained from the screening using RAPD marker analysis.A total of 202 bands were obtained;167 of them showed polymorphisms,with a polymorphism rate of 82.67%;the genetic similarity（GS）coefficients among the studied cultivars ranged from 0.613 to 0.870;the average genetic similarity coefficient was 0.744.A total of 297 bands were amplified from these 91 pairs of SSR primers,and 267 bands showed polymorphisms,with a polymorphism rate of 89.90%,and genetic similarity coefficients ranging from 0.555 to 0.837 among the 20 sweet sorghum varieties were obtained,and the average value was 0.695.This result displayed a similar trend with results from the 2 individual data analyses discussed above for RAPD and SSR molecular markers,with some differences.The results based on combined data could more comprehensively and objectively reflect the fundamental genetic differences among these varieties.

关 键 词：甜高粱 聚类分析 SSR RAPD 多态性 

分 类 号：S514.03[农业科学—作物学]