控制白菜叶片紫色的pur基因初步定位  

Primary Mapping of pur,a Gene Controlling Purple Leaf Color in Brassica rapa

作　　者：刘瑾[1,2] 汪维红[2] 张德双[2] 于拴仓[2] 张凤兰[2] 赵岫云[1,2] 余阳俊[2] 徐家炳[2] 卢桂香[2] 

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室,北京100097

出　　处：《华北农学报》2013年第1期49-53,共5页Acta Agriculturae Boreali-Sinica

基　　金：科技部“973”项目(2012CB113906);北京市科技项目(D111100001311002);国家科技支撑计划(2012BAD02B01);北京市自然科学基金项目(6092009);大宗蔬菜产业技术体系(CARS-25-A-11);北京市留学人员科技活动择优资助项目(优秀类)

摘　　要：为了定位控制白菜叶片紫色的pur基因,选用大白菜自交系09-680和紫色小白菜09N-742进行杂交构建了一个由307个单株组成的F2群体,采用群体分离分析法(Bulked segregant analysis,BSA)构建紫色和绿色池,对分布在白菜基因组10个连锁群上的125个InDel标记和100个SSR标记进行多态筛选,其中位于A3连锁群末端的2个In-Del标记BrID10999和BrID10399与紫色性状表现连锁。连锁分析发现,2个标记与pur基因的遗传距离分别为7.3,5.7 cM,位于pur基因的同侧。在此基础上,根据这些标记所在区域的BAC序列设计了23对SSR引物,其中来源于KBrH005P10的SSR标记BVRCP10-6位于pur基因的另一侧,距离pur基因仅1.9 cM。这些标记可有效用于白菜紫色性状的分子标记辅助育种,也为进一步精细定位和克隆pur基因奠定了基础。In order to map the pur gene controlling purple leaf color in Brassica rapa,a F2 population including 307 individuals was constructed by crossed Chinese Cabbage inbred line 09-680（green leaf）with non-heading Chinese Cabbage inbred line 09N-742（purple leaf）.A bulk segregant analysis（BSA）technology was conducted by screening 125 PCR-based insertion/deletion（InDel）markers and 100 simple sequence repeate（SSR）markers distributed on 10 linkage groups to screen the polymorphism between the purple and green leaf pools.Of these,two InDel markers BrID10999 and BrID10399 were found to be linked to the pur gene.By linkage analysis,two markers were located at the end of A3,at the same side of pur gene at distances of 7.3,5.7 cM,respectively.In order to obtain tightly linked markers on both sides of pur gene,23 SSR primers derived from three bacterial artificial chromosome（BAC）clones were selected,of which one SSR marker BVRCP10-6 from KBrH005P10 was indentified as closely linked to pur gene at a genetic distance of 1.9 cM,at the other side of the pur gene.These markers could be very helpful for marker-assisted selection（MAS）in purple Chinese cabbage hybrid breeding programs as well as for fine mapping and cloning this gene.

关 键 词：大白菜 叶片颜色 PUR 基因定位 

分 类 号：S634.03[农业科学—蔬菜学]