莫西沙星经ERK1/2通路抑制铜绿假单胞菌诱导气道上皮细胞产生MUC5AC  

Moxifloxacin attenuate Pseudomonas aeruginosa-induced MUC5AC expression by inhibition of ERK1/2 phosphorylation in airway epithelial cells

作　　者：谢莉[1] 谭小武[1] 曾赛丽[1] 何振华[1] 周洲[2] 游晓星[2] 

机构地区：[1]南华大学附属第二医院呼吸内科,湖南省衡阳市421001 [2]南华大学医学院病原生物学研究所,湖南省衡阳市421001

出　　处：《实用医学杂志》2013年第7期1041-1044,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:30901352)

摘　　要：目的:研究喹诺酮类抗生素莫西沙星(moxifloxacin,MXF)对铜绿假单胞菌(Pseudomonas aeruginosa,PA)诱导气道上皮细胞表达产黏蛋白MUC5AC的影响,并探讨其可能的分子机制。方法:体外培养NCI-H292细胞,用不同浓度的PA、产藻酸盐型PA脂多糖(光滑型,sPA-LPS)、非藻酸盐型PA脂多糖(粗糙型,rPA-LPS)刺激,然后加入不同浓度MXF孵育24h,分别采用ELISA和实时定量PCR检测MUC5AC蛋白和mRNA的表达情况,Westernblot检测NCI-H292细胞内ERK1/2的激活情况。结果:PA、sPA-LPS和rPA-LPS能明显诱导NCI-H292细胞产生和表达MUC5AC,并能促进ERK1/2磷酸化。而MXF处理后,能以剂量依赖性方式抑制PA、sPA-LPS或rPA-LPS诱导的MUC5AC蛋白表达,并有效抑制ERK1/2的磷酸化。结论:MXF能抑制PA诱导的黏蛋白表达,因此有望用于PA诱导的黏蛋白过度分泌的治疗。Objective To investigate the effect of fluoroquinolone＇s antibiotics moxifloxacin （MXF） on Pseudomonas aeruginosa （PA）-induced mucus MUCSAC expression in airway epithelial cells. Methods NCI-H292 cells were stimulated with PA, lipopolysaccharide from alginate producing PA （smooth, sPA-LPS） and non-alginate producing PA （rough,rPA-LPS） respectively for 24 h in vitro. MXF was added in different concentrations. Expressions of MUC5AC gene and mucin protein was quantified by ELISA and real time PCR respectively, while phosphorylation of ERK1/2 was detected by Western blot. Results PA, sPA-LPS or rPA-LPS could significantly induce MUC5AC gene and protein expression in NCI-H292 cells,also phosphorylate ERK1/2,and MXF could significantly inhibit it. Conclusion MXF can attenuate PA-induced mucus expression in human airways and may be used for treating PA-induced mucus hypersecretion in clinical practice.

关 键 词：假单胞菌 铜绿 莫西沙星 ERK1 2 MUC5AC 

分 类 号：R96[医药卫生—药理学]