对比评价紫檀芪和白藜芦醇的抗辐射活性  

Comparative Evaluation of Anti-radiation Effect of Pterostilibene and Resveratrol

作　　者：白佳利[1] 于程程[1] 王德芝[1] 郝建秀[1] 沈秀[1] 王浩[1] 刘晓秋[1] 龙伟[1] 周则卫[1] 

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所,天津300192

出　　处：《医药导报》2013年第4期415-418,共4页Herald of Medicine

基　　金：国家自然科学基金(81202153);协和青年基金(2012D03);中国医学科学院放射医学研究所发展基金(SF1227)

摘　　要：目的对比评价等摩尔当量紫檀芪和白藜芦醇对ICR小鼠的抗辐射作用。方法以6～8周龄ICR雄性小鼠为研究对象,给予6.5 Gyγ-射线一次性全身照射。空白照射组(给予0.5%羧甲基纤维素钠溶液),阳性对照组(给予茜草双酯100 mg.kg-1),白藜芦醇低、高剂量组(分别给予白藜芦醇50,150 mg.kg-1),紫檀芪低、高剂量组(分别给予紫檀芪56,168 mg.kg-1),均在照射前3 d内连续3次灌胃给药,照射后连续给予5 d,并在照射7 d后眼眶取血,解剖取胸腺、肝、脾、肺及两侧股骨。称重计算相关脏器系数,血清生化分析仪测白细胞计数(WBC)、血小板计数(PLT),以及股骨有核细胞(BMNC)和骨髓DNA,考察目标化合物紫檀芪和白藜芦醇的抗辐射损伤效应。结果与空白对照组比较,各实验组的各项指标均有所增加,且大部分差异有统计学意义(P<0.05),相同摩尔当量的紫檀芪实验组的抗辐射活性优于白藜芦醇组,尤其是紫檀芪低剂量组效果最佳,与白藜芦醇低剂量组比较差异有统计学意义(P<0.05)。结论紫檀芪对6.5Gy辐射损伤ICR小鼠的防护作用优于白藜芦醇,且紫檀芪低剂量组辐射防护效果最佳。Objective To compare the anti-radiation effect between pterostilibene and resveratrol in equimolar oral doses on ICR mice. Methods ICR mice of 6 - 8 weeks were selected to receive whole body irradiation of 6.5 Gy γ-ray. The mice were randomly divided into six groups to receive the following treatment：Control group, 0.05% CMC-Na; positive control group, rubidate 100 mg · kg^-1 ; low-and high-dose resveratrol groups, resveratrol at 50 or 150 mg · kg^-1 ; low-and high-dose pterostilibene groups, pterostilibene at 56 or 168 mg · kg^-1. The mice of the above groups were administered intragastrically 3 days, 2 days, and 1 day before the radiation. After the radiation, the mice were administered intragastrically for 5 consecutives days. The thymus, liver, spleen, lung and bilateral femurs were weighed to calculate corresponding organ indexes. White blood cell count （WBC）, blood platelet （PLT）, bone marrow nucleated cells （BMNC） and bone marrow DNA were determined, so as to evaluate the radiation-protective effect of pterostilibene and resveratrol. Results Compared with control mice, the indicators of all experiment groups were increased and most have significant difference （P〈0.05） ; the anti-radiation effect of pterostilibene was better than resveratrol at the same equimolar oral dose, with significant difference between the low-dose pterostilibene （56 mg · kg^-1 ） group and the low-dose resveratrol （50 mg · kg^-1 ） group. Conclusion Pterostilibene has better anti-radiation effect on ICR mice than resveratrol, and the anti-radiation effect of pterostilibene at 56 mg · kg^-1 is the best.

关 键 词：紫檀芪 白藜芦醇 辐射防护 

分 类 号：R965[医药卫生—药理学]