牛痘疫苗致炎兔皮提取物注射液对大鼠脑挫伤皮质细胞凋亡基因Bcl-2m RNA表达的影响  

Effect of Analgecine on mRNA Expression of Bcl-2 in Perienchyma of Cerebral Contusion Focus

作　　者：胡瑾[1] 张晓丽[2] 张辉[2] 

机构地区：[1]河北北方学院第一附属医院神经内科,张家口075029 [2]河北北方学院基础医学院,张家口075029

出　　处：《医药导报》2013年第4期435-437,共3页Herald of Medicine

摘　　要：目的探讨脑挫伤灶周围区凋亡基因Bcl-2 mRNA表达及牛痘疫苗致炎兔皮提取物注射液(恩再适)对其干预作用。方法采用Feeny's自由落体法复制大鼠脑挫伤动物模型。牛痘疫苗致炎兔皮提取物注射液治疗24,72 h取脑切片,采用原位杂交与医学图像分析技术检测Bcl-2 mRNA阳性细胞数目、平均灰度值。结果治疗组Bcl-2 mRNA阳性细胞数目和深度高于对照组和模型组(P<0.05),72 h组明显高于24 h治疗组(P<0.05)。结论牛痘疫苗致炎兔皮提取物注射液对脑挫伤皮质细胞有保护作用,并能明显上调Bcl-2 mRNA的表达,从而减轻神经元的损伤。Objective To investigate mRNA expression of Bcl-2 in perienchyma of cerebral contusion focus and effect of analgecine on it. Methods The cerebral contusion rat model was established by Feeney＇s falling body injury and treated with analgecine. The brain was taken and sectioned 24 and 72 h after the treatment. Bcl-2 mRNA positive cells were determined by using in situ hybridization and medical image analysis technique. Results The number of Bcl-2 mRNA positive cells and the average gray value of the treatment group were significantly higher than those of sham operated group and model group （P〈0.05）. Those were significantly higher in the 72 h group than in the 24 h group （P〈0.05）. Conclusion Analgecine could protect the neurons from cerebral contusion. It can promote expression of Bcl-2 mRNA and alleviate neuron injury in penumbra area.

关 键 词：牛痘疫苗致炎兔皮提取物注射液 挫伤 脑 原位杂交 

分 类 号：R965[医药卫生—药理学]