葛根与粉葛饮片的色谱指纹图谱研究  

Studies on Chromatographic Fingerprints of Pueraria lobata and Pueraria thomsonii

作　　者：王金华[1] 姚仲青[2] 

机构地区：[1]南京市高淳县中医院药剂科,南京211300 [2]扬子江药业集团有限公司中药研究院,江苏泰州225321

出　　处：《医药导报》2013年第4期525-529,共5页Herald of Medicine

摘　　要：目的建立葛根与粉葛质量比较的色谱方法,并用于二者的质量分析与评价,为其质量控制提供新依据,为其药效物质基础的分析奠定基础。方法采用薄层色谱(TLC)法对7个葛根样品和17个粉葛样品进行比较,并采用高效液相色谱(HPLC)法进行指纹图谱测定,运用中药色谱指纹图谱相似度评价系统(2004A)软件进行分析。采用对照品对比和HPLC-MS技术对其部分色谱峰进行定性鉴别。结果建立的TLC和HPLC指纹图谱能区别葛根和粉葛。HPLC指纹图谱粉葛饮片共有峰14个,野葛根饮片共有峰19个,初步指认了葛根与粉葛HPLC指纹图谱中13和7个成分。结论该文为葛根与粉葛质量控制和临床应用提供可靠依据。Objective To establish chromatographic fingerprints for the identification and quality evaluation of Pueraria lobata and Pueraria thomsonii. Methods Seventeen samples of Pueraria thomsonii and 7 samples of Puerar/a lobata were analyzed with the thin-layer chromatography （TLC） and the developed high performance liquid chromatography （HPLC） fingerprint method. The HPLC data was calculated with similarity evaluation system for chromatographic fingerprint of TCM （ Version 2004A）. Results A satisfactory fingerprint was obtained by HPLC method, and qualitative analyses of some of the peaks were carried out by HPLC/MS approach. Conclusion The present research provides useful evidence for the quality control of evaluation of Pueraria lobata and Pueraria thomsonii.

关 键 词：葛根 粉葛 色谱指纹图谱 

分 类 号：R282.71[医药卫生—中药学]