人参发状根与栽培人参有效成分的比较研究  

Study on Comparison of Physiological and Biochemical Composition Between Hair Root of Ginseng and Cultivated Ginseng

作　　者：盖玉红[1] 王文慧[1] 王沥浩[1] 魏健[2] 

机构地区：[1]吉林农业大学,吉林长春130118 [2]长春师范学院生命科学学院,吉林长春130118

出　　处：《北方园艺》2013年第7期166-169,共4页Northern Horticulture

基　　金：吉林省教育厅科研资助项目(吉教科合字[2012]第59号;吉教科合字[2011]第191号)

摘　　要：为了研究人参发状根这种有别于人参的特殊器官与人参之间的关联,采用考马斯亮蓝法测定了人参各部位的蛋白含量,同时采用标准曲线法测定了人参发状根与其它不同部位的皂苷含量、游离氨基酸含量、可溶性糖含量、过氧化物酶含量和丙二醛含量等各项生理生化指标,并与栽培人参根的各部位进行比较。结果表明:人参发状根中的皂苷含量高于栽培人参中的皂苷含量,且以人参芦头和人参须根的皂苷含量最高;固体培养的人参发状根和液体培养的人参发状根的皂苷含量相比,尤以液体培养的人参发状根的皂苷含量最高,可达3.05%,发状根的丙二醛MDA与栽种人参芦头和人参须根中的MDA均偏低。To study the association between ginseng and specific organ hairy root of ginseng which is different from ginseng, the protein contents in every part of ginseng were determined by coomassie brilliant blue method,and the saponin content,free amino acid content, soluble sugar content, peroxidase and malondialdehyde content in ginseng root and other hairy parts were determined using the standard curve method; the saponin content in hair root of ginseng and other physiological and biochemical indexes were determined and compared with every part in cultivated ginseng. The results showed that the saponin content in hairy roots of ginseng was higher than in liquid culture,and the saponin contents in rhizoma and fibrous roots were the highest. It indicated that in liquid and solid culture, the saponin content in hair root was as same as or more higher than in root, especially the saponin content in liquid culture reached the highest, up to 3. 05% ,and the MDA content in hair root of ginseng was lower than in rhizome and fibrous roots.

关 键 词：人参发状根 固体培养 液体培养 人参皂苷 

分 类 号：S567.51[农业科学—中草药栽培]