红霉素对产甲烷菌的抑制及其驯化  

Inhibition of Methanogenium by Erythromycin and Its Domestation

作　　者：刘子旭[1,2] 孙力平[1,2] 李玉友[1,2,3] 邱春生[1,2] 

机构地区：[1]天津城市建设学院环境与市政工程学院,天津300384 [2]天津市水质科学与技术重点实验室,天津300384 [3]日本东北大学环境科学研究科

出　　处：《环境科学》2013年第4期1540-1544,共5页Environmental Science

基　　金：国家水体污染控制与治理科技重大专项(2012ZX07308-002)

摘　　要：红霉素是一类具有一定生物毒性的抗生素类药品,为探明红霉素对产甲烷菌的抑制作用及其可驯化能力,依次在厌氧瓶中进行厌氧毒性试验、在升流式厌氧污泥床反应器(UASB)中进行连续实验,测定累计甲烷产量、相对产甲烷速率、COD去除率、甲烷含量.结果表明,红霉素为150 mg.L-1时产甲烷速率降为56.1%;250 mg.L-1时反应速率降低99%以上,活性受到完全抑制.保持红霉素投加量为20 mg.L-1连续运行60 d,COD去除率、甲烷含量可达到81.4%、64.2%.红霉素对甲烷菌有抑制作用,半抑制浓度为150 mg.L-1.甲烷菌对红霉素有一定的驯化能力,驯化60 d后COD去除率、甲烷含量较未驯化时可提高15.13%、22.05%.Erythromycin is a kind of antibiotic drugs with certain biological toxicity.In order to investigate the inhibitory effect of erythromycin on methanogens and its acclimation capacity,Anaerobic Toxicity Assay（ATA） and continuous experiment were conducted in anaerobic bottles and the Up-flow Anaerobic Sludge Blanket Reactor（UASB）,respectively,to determine the accumulated methane production,ratio of methane production rate,COD removal efficiency,and methane content.The results showed that the methane production ratio was reduced to 56.1% in the presence of 150 mg·L-1 of erythromycin and it was reduced by 99% when the erythromycin reached 250 mg·L-1,indicating that the activity was completely inhibited.Keeping the erythromycin at an concentration of 20 mg·L-1in the process of continuous operation for 60d,the COD removal efficiency and methane content reached up to 81.4% and 64.2%,respectively.The results suggested that erythromycin had an inhibitory effect on methane bacteria,and the half inhibitory concentration was 150 mg·L-1（IC50：150 mg·L-1）.The COD removal efficiency and methane content were increased by 15.13% and 22.05%,respectively,after domestication for 60 d.

关 键 词：红霉素 产甲烷菌 产甲烷速率 抑制 甲烷含量 

分 类 号：X703[环境科学与工程—环境工程]