王氏连朴饮加味对脾胃湿热证大鼠抗氧化功能的影响  

Effect of Jiawei Wangshi Lianpu Drink on Antioxidation of Rats with Splenogastric Damp-heat Syndrome

作　　者：张霓[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《河南中医》2013年第4期519-520,共2页Henan Traditional Chinese Medicine

摘　　要：目的:观察王氏连朴饮加山楂、桃仁对脾胃湿热证模型大鼠红细胞氧化物歧化酶(SOD)、丙二醛(MDA)及血清谷胱甘肽过氧化物酶(GSH-Px)、D-木糖含量的影响。方法:将32只Wistar大鼠随机分为正常对照组、模型组、王氏连朴饮治疗组、王氏连朴饮加山楂、桃仁治疗组各8只,采用内外因湿热证模型综合造模方法建立脾胃湿热证大鼠模型,共10 d。造模成功后开始灌胃给药:正常对照组:灌服生理盐水1.3 mL.(kg.d)-1)[2.6 mL.(只.d)-1];脾胃湿热证模型组:灌服生理盐水1.3 mL.(kg.d)-1[2.6 mL.(只.d)-1];王氏连朴饮治疗组:灌服王氏连朴饮1.3 mL.(kg.d)-1[2.6 mL.(只.d)-1];王氏连朴饮加山楂、桃仁治疗组:灌服王氏连朴饮加山楂、桃仁汤1.3 mL.(kg.d)-1[2.6 mL.(只.d)-1]。5 d后处死大鼠,采用邻苯三酚自氧化法检测红细胞SOD含量,改良八木国夫法检测MDA含量,甲苯胺改良法检测GSH-Px含量,比色法检测D-木糖含量。结果:脾胃湿热证模型大鼠红细胞SOD显著降低,MDA明显升高(P<0.01),血清GSH-Px及D-木糖显著降低(P<0.01)。经王氏连朴饮加山楂、桃仁治疗后SOD含量升高正常,MDA含量恢复正常,GSH-Px及D-木糖含量恢复正常,与正常对照组比较无统计学意义(P>0.05)。结论:王氏连朴饮加山楂、桃仁能有效改善脾胃湿热证大鼠诸症状,使降低的SOD、GSH-Px及D-木糖升高至正常。使升高的MDA降至正常。其作用机理可能是通过调节大鼠抗氧化功能而起效。Objective： To observe the effect of Wangshi Lianpu Drink plus hawthorn and peach kernel on content of erythrocyte superoxide dismutase（SOD）,malondialdehyde（MDA） and serum glutathione peroxidase（GSH-Px）,D-xylose of model rats with splenogastric damp-heat syndrome.Methods： 32 Wistar rats were randomly divided into normal control group,model group,Wangshi Lianpu Drink treatment group,Wangshi Lianpu Drink plus hawthorn and peach kernel treatment group,with 8 rats in each group.The model rats with splenogastric damp-heat syndrome were established with the integrated modeling method of internal and external causes of damp-heat syndrome for 10 d.The intragastric administration began after the modeling.The normal control group and the splenogastric damp-heat syndrome model group were given normal saline 1.3 mL·（kg·d）-1（2.6 mL/ /d）;Wangshi Lianpu Drink treatment group were fed with Wangshi Lianpu Drink 1.3 mL·（kg·d）-1（2.6 mL/ /d）;Wangshi Lianpu Drink plus hawthorn and peach seed treatment group were fed with Wangshi Lianpu Drink plus hawthorn and peach kernel decoction 1.3 mL·（kg·d）-1（2.6 mL/ /d）.The model rats were killed 5 D later.The erythrocyte SOD content was detected with pyrogallic acid oxidation method,MDA content with modified hachikokufu method,GSH-Px content with modified toluidine method,D-xylose content with colorimetric method.Results： In the splenogastric damp-heat syndrome model rats,erythrocyte SOD content decreased significantly,MDA content increased significantly（P＆lt;0.01）,the content of serum GSH-Px and D-xylose decreased significantly（P＆lt;0.01）.After the treatment with Wangshi Lianpu Drink plus hawthorn,peach kernel,SOD content increased normally,the content of MDA,GSH-Px and D-xylose returned to normal,there was no statistical significance（P＆gt;0.05） compared with the normal control group.Conclusion： Wangshi Lianpu Drink plus hawthorn,peach kernel can effectively improve the symptoms of rats with splenogastric damp-heat syndrome,ma

关 键 词：王氏连朴饮 脾胃湿热证 氧化物歧化酶 丙二醛 谷胱甘肽过氧化物酶 D-木糖 大鼠 

分 类 号：R285.5[医药卫生—中药学]