丁香酚对小麦-蚕豆间作土壤微生物数量及多样性的影响  

Effects of eugenol on soil microbes in wheat faba-bean intercropping and mono-cropping systems

作　　者：刘辉娟[1] 柴强[1] 黄高宝[1] 周海燕[1] 朱静[1] 

机构地区：[1]甘肃省干旱生境作物学重点实验室甘肃农业大学农学院,甘肃兰州730070

出　　处：《草业科学》2013年第3期346-351,共6页Pratacultural Science

基　　金：国家自然科学基金项目(31160265);甘肃省杰出青年基金;国家公益性行业项目(201103001)

摘　　要：通过盆栽试验,探讨了在田间持水量75%水平下,小麦(Triticum aestivum)根系分泌物丁香酚对小麦、蚕豆(Vicia sativa)单作和间作群体土壤微生物数量及其多样性的影响,以期为化感物质间作群体的调控提供理论依据。结果表明,总体上,微生物的多样性降低,真菌和放线菌数量减少,而细菌数量增加。与无丁香酚处理对比,在3种种植模式中,丁香酚对土壤真菌和放线菌数量均表现为化感抑制作用,两种微生物数量的减小幅度分别为21.95%～98.42%和13.33%～92.82%;单作小麦较间作处理的细菌数量变化了-118.63%～90.73%,但单作蚕豆细菌数量变化了-56.39%～76.76%;放线菌占微生物的相对数量变化了-4.42%～4.32%,细菌变化了-4.36%～4.88%。小麦间作蚕豆降低了土壤微生物多样性指数,较单作小麦、单作蚕豆分别变化了15%～26.98%和-45.45%～6.67%;经丁香酚处理,单作中土壤微生物多样性指数降低,而间作变化不大。In order to provide theoretical basis for the regulation of intercropping system of existed allelochemicals, effects of eugenol, a kind of wheat root-exudate, on microbial population and diversity of inter- cropped and mono-cropped wheat faba-bean systems under 75 % field capacity condition were investigated by a pot experiment. Results showed that as a whole, soil microbial diversities decreased, fungi and acti- nomycetes population decreased, and bacteria population increased in wheat-faba bean intercropping and mono-cropping systems. Among the three cropping patterns, compared with no eugenol application, eugenol treatments displayed inhibitory effects on the amounts of soil fungi and actinomycetes, which decreased by 21.95%-98.42% and 13.33%-92.82%, respectively. Bacterial population of sole wheat decreased by -118.63%-90. 73% than intercropping treatments, while that of sole bean increased by -56.39%- 76.76%. The relative amount of actinomycetes of the microbial population declined by -4.42%-4.32%, while the ratio of bacterial population increased by -4. 36%-4. 32%. Compared with wheat and bean monoculture, wheat-bean intercropping could reduce soil microbial diversity index, in which soil microbial diversity index decreased by 15%-26.98% and -45.45%-6.67%, respectively. The microbial diversity index of monoculture with eugenol treatment declined.

关 键 词：丁香酚 种植模式 土壤微生物 化感作用 

分 类 号：S154.3[农业科学—土壤学]