UPLC法同时测定不同产地知母药材中新芒果苷和芒果苷的含量  

Simultaneous Determination of Neomangiferin and Mangiferin in Anemarrhena asphodeloides from Different Habitats by UPLC

作　　者：钟可[1,2] 王文全[1,3,4] 靳凤云[2] 侯俊玲[1] 刘进涛[2] 赵志刚[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]贵阳中医学院,贵阳550001 [3]中国医学科学院/北京协和医学院药用植物研究所,北京100193 [4]中药材规范化生产教育部工程研究中心,北京100102

出　　处：《中国药房》2013年第15期1386-1388,共3页China Pharmacy

基　　金：国家公益性(中医药)行业科研专项资助项目(No.201107009-03)

摘　　要：目的:建立同时测定知母药材中新芒果苷和芒果苷含量的方法,并测定不同产地知母中新芒果苷和芒果苷的含量,评价不同产地知母药材的质量。方法:采用超高效液相色谱(UPLC)法。色谱柱为ACQUITYUPLCBEHC18(100mm×2.1mm,1.7μm),流动相为0.4%冰醋酸水-甲醇(梯度洗脱),流速为0.25ml/min,柱温为35℃,检测波长为258nm。结果:新芒果苷和芒果苷的进样量分别在0.0300～0.4800、0.0285～0.4560μg范围内与各自峰面积积分值呈良好的线性关系(r均为0.9999);精密度、重复性、稳定性试验的RSD均<3%;平均加样回收率分别为97.67%、100.17%,RSD分别为2.87%、2.78%(n均为6)。结论:该方法快速、准确,可用于同时测定知母中新芒果苷和芒果苷的含量。不同产地的知母质量存在差异。OBJECTIVE： To establish a method for simultaneous determination of neomangiferin and mangiferin in A nemarrhena asphodeloides and determine the contents of two components in A. asphodeloides from different habitats, and to evaluate the quality of A. asphodeloides from different habitats. METHODS： UPLC method was adopted. The determination was performed on ACQUITY UPLC BEH C18（100mm×2.1mm,1.7μm） column with mobile phase consisted of 0.4% acetic acid-methanol （gradient elution） at the flow rate of 0.25 ml/min. The column temperature was 35 if2 and the detection wavelength was set at 258 nm. RESULTS： The linear ranges of neomangiferin and mangifer were 0.030 0-0.480 0 pg and 0.028 5-0.456 0 /ag（r：0.999 9）.RSDs of precision test, reproducibility test and stability test were all lower than 3%. The average recoveries were 97.67 % （RSD= 2.87 %, n=6） and 100.17%（RSD：0.78% ,n=6）, respectively. CONCLUSION： The method is rapid and accurate, and can be used for simultaneous determination of neomangiferin and mangiferin in A. asphodeloides. The qualities ofA. asphodeloides from different habitats are different.

关 键 词：知母 超高效液相色谱法 芒果苷 新芒果苷 不同产地 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]