川芎的UPLC指纹图谱研究  

Study on UPLC Fingerprints of Ligusticum chuanxiong

作　　者：马天成[1] 崔思娇[1] 张靓琦[1] 范旭航[1] 毕开顺[2] 贾英[1] 

机构地区：[1]沈阳药科大学中药学院,沈阳110016 [2]沈阳药科大学药学院,沈阳110016

出　　处：《中国药房》2013年第15期1389-1391,共3页China Pharmacy

基　　金：辽宁省教育厅高等学校科研项目(No.2009T097)

摘　　要：目的:建立川芎的超高效液相色谱(UPLC)指纹图谱。方法:色谱柱为ACQUITYUPLCHSST3(100mm×2.1mm,1.8μm),流动相为甲醇-0.03%磷酸水(梯度洗脱),检测波长为274nm,流速为0.35ml/min,柱温为30℃。结果:首次建立了川芎的UPLC指纹图谱共有模式,标定了18个共有峰,结合保留时间和紫外光谱分析,指认了咖啡酸、香草醛、阿魏酸、洋川芎内酯Ⅰ、洋川芎内酯H、丁基苯酞、藁本内酯和丁烯基苯酞的峰位。14批川芎药材中有12批的相似度在0.900以上。结论:该方法快速、高效,可用于川芎的质量评价。OBJECTIVE： To establish UPLC fingerprint of Ligusticum chuanxiong. METHODS： The determination was performed on ACQUITY UPLC HSS T3 （100mm×2.1mm,1.8μm） column with mobile phase consisted of methanol-0.03 % phosphoric acid （gradient elution） at the flow rate of 0.35 ml/min. The detection wavelength was set at 274 nm, and column temperature was 30 ℃. RESULTS： The common mode of UPLC fingerprint ofL. chuanxiong was set up firstly. There were 18 common peaks in the fingerprints. Caffeic acid, vanillin, ferulic acid, senkyunolide I , senkyunolide H, butylphthalide, ligustilide and butylidenephthalide were identified by comparing the retention time and their ultraviolet spectra. The similarity of 14 batches of L. chuanxiong was more than 0.900, except for 2 batches of samples. CONCLUSION： The method is rapid and efficiency. It can be used for the quality evaluation of L. chuanxiong.

关 键 词：川芎 超高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学] R917[医药卫生—中医学]