鼠曲草饮片的RP-HPLC指纹图谱研究  

Study on RP-HPLC Fingerprints of Gnaphalium affine Decoction Pieces

作　　者：赵颖[1] 李文[1] 李鹏 芮海波[1] 谈瑄忠[1] 

机构地区：[1]南京市中医院,南京210001 [2]南京海昌中药集团有限公司,南京210061

出　　处：《中国药房》2013年第15期1391-1393,共3页China Pharmacy

基　　金：南京市科技发展计划资助项目(No.201007005)

摘　　要：目的:建立鼠曲草饮片的反相-高效液相色谱(RP-HPLC)指纹图谱。方法:以绿原酸和异鼠李素为内参照物,色谱柱为YMC-PackODS-A(250mm×4.6mm,5μm),流动相为0.1%磷酸水溶液-乙腈(梯度洗脱),流速为1.0ml/min,检测波长为320mn,柱温为35℃。结果:在RP-HPLC指纹图谱中确立了20个共有峰,建立了鼠曲草饮片的共有模式。10批鼠曲草饮片的相似度均大于0.900。结论:该方法简便、准确、重复性好,可为鼠曲草饮片的质量评价提供依据。OBJECTIVE： To establish RP-HPLC fingerprint of Gnaphalium affine decoction pieces. METHODS： Using chlorogenie acid and isorhamnetin as reference substance, the determination was performed on YMC-PackODS-A（250mm×4.6mm,5μm） column with mobile phase consisted of 0.1%phosphate acid-acetonitrile （gradient elution） at the flow rate of 1.0 ml/min. The detection wavelength was set at 320 nm, and the column temperature was 35 ℃. RESULTS： In RP-HPLC fingerprint, 20 common peaks were identified, and common model of G. affine tablets was established. The similarities of 10 batches were exceeded 0.9. CONCLUSION： The method is simple, accurate and reproducible, and it provide reference for quality control of G. aJfine decoction pieces.

关 键 词：鼠曲草 指纹图谱 反相-高效液相色谱法 绿原酸 异鼠李素 

分 类 号：R284.1[医药卫生—中药学] R917[医药卫生—中医学]