模拟干旱条件下水稻苗期形态性状的QTL定位  

QTL Mapping for Rice Seedling Morphological Traits under Simulated Drought Stress Conditions

作　　者：刘宇强[1] 赵宏伟[1] 王敬国[1] 刘化龙[1] 王晋[1] 贾琰[1] 

机构地区：[1]东北农业大学农学院水稻研究所,黑龙江哈尔滨150030

出　　处：《作物杂志》2013年第2期42-48,共7页Crops

基　　金：国家科技支撑项目"东北平原北部(黑龙江)春玉米水稻持续丰产高效技术集成创新与示范"(2011BAD16B11);黑龙江省"十二五"科技攻关项目"高产优质节水栽培技术研究"(GA10B102-5)

摘　　要：以抗旱性差异较大的亲本小白粳子和空育131以及其后代180个F2∶3家系群体为试验材料,构建了包含99个SSR分子标记的遗传连锁图谱,利用浓度为15%PEG-6000模拟干旱胁迫条件。在两种条件下,共检测到影响胚芽鞘长、苗高、主根长和根数的QTL 26个,分别位于水稻的第1、2、3、4、5、6、7、8、12条染色体上,贡献率变幅在4.53%～37.22%之间。其中控制苗高的QTL11个,控制胚芽鞘长的QTL 5个,控制主根长的QTL 5个,控制根数的QTL 5个。在第2条染色体的RM1358-RM1347区间和第6条染色体上的RM461-RM162区间发现了控制多个性状的QTL,在第2条染色体的RM1358-RM1347区间和第8条染色体的RM1384-RM547区间还检测到了在两种条件下同时控制胚芽鞘长和主根长的QTL。A F2：3 group derived from the cross of Xiaobaijingzi/Kongyu 131 ,which both parents were significantly different in drought resistance,was used in QTL analysis of coleoptile length, seeding height, main-root length and root number under 15% PEG-6000 simulated drought stress. A total of 26 QTL related seedling drought resistance characteristics located to chromosome 1,2,3,4,5,6,7,8 and 12, respectively were detected from the molecular linkage map comprised 99 SSR markers. Among them,5 QTLs for coleoptile length,5 QTLs for main-root length,5 QTLs for root number, with contributions ranging from 4.53 % - 37.22%. We found QTL on chromosome 2 delimi- ted by SSR marker RM 1358 and RM 1347, QTL on chromosome 8 flanked by RM1384 and RM547, and QTL on chromosome 6 flanked by RM461 and RM162 had effects on multiple characters. In addition,the QTLs on chromo- some 2 and chromosome 8 can control coleoptile length under two different conditions.

关 键 词：水稻 耐旱 苗期 QTL 定位 

分 类 号：S644.6[农业科学—蔬菜学]