针对组织工程多孔生物陶瓷的组织学技术优化探讨  

Technique improvement of hard tissue slicing of bioceramic scaffold materials applied in bone tissue engineering

作　　者：李林[1] 智伟[1] 桑力[1] 张成栋[1] 李金雨[1] 张聪[2] 娄延举[2] 夏天[2] 翁杰[1] 

机构地区：[1]西南交通大学材料学院材料先进技术教育部重点实验室,成都610031 [2]中国人民解放军第四五二医院,成都610021

出　　处：《国际生物医学工程杂志》2013年第1期14-16,I0005,共4页International Journal of Biomedical Engineering

摘　　要：目的改进硬组织切片技术以适应生物陶瓷材料在骨组织工程中的研究。方法探索硬组织切片的厚度、漂片温度、裱片方法、烤片温度和时间的最佳组合，针对阳离子防脱载玻片的使用条件进行反复比较，通过改进操作流程中的关键技术和需避免的问题，摸索出阳离子载玻片在硬组织切片裱片中的最佳应用条件，克服了硬组织切片制作技术中标本易破碎、切片易脱落及染色时染料容易吸附的缺点。结果通过技术探索与改进，生物陶瓷支架材料体内植入后的类骨修复体标本的硬组织切片能保持其杂化后的组织结构与比较完整的材料结构，可进行Masson三色染色、苏木精一伊红（HE）及甲苯胺蓝染色。染色后镜下观察显示支架内杂化生长的组织结构完整、细胞形态清晰、切片质量好、生物陶瓷支架脱片少。荧光显微镜可观察到类骨修复体钙沉积现象完整。结论改善了传统硬组织切片技术处理生物陶瓷材料时易于破坏组织一材料结构的缺点。改进的硬组织切片技术适应生物陶瓷材料在骨组织工程领域研究。Objective Purpose To improve the hard tissue slicing technology to adapt to the study of the bioceramic materials in bone tissue engineering. Methods Purpose To improve the hard tissue slicing technology to adapt to the study of the bioceramic materials in bone tissue engineering. Results The improved techniques in hard tissue slicing could keep the morphosis and structure of hybrid tissues, and easily stain with Masson, HE and toluidine blue. The stained hard tissue slicing had an intact tissue structure, clear cell form, good slicing quality, little shedding. Fluorescence microscope showed an intact calcium deposition of homologous bone restoration. Conclusion The method overcome the shortcomings of easy to destroy the tissue-material structure happened in the traditional hard tissue slicing of bioceramic materials. Conclusion The improved technique in hard tissue slicing is more useful than traditional technique and can be adapted to the application of bioceramic materials in bone tissue engineering.

关 键 词：硬组织切片 生物陶瓷材料 阳离子载玻片 骨组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]