ETEC K99、K88菌毛蛋白抗原表位多肽的设计合成及免疫反应  

Design Synthesis and Immunological Reaction of Epitope Peptides of ETEC K88 and K99 Fimbrial Protein

作　　者：余丽芸[1] 田斌[1] 温丽娟[1] 王桂华[1] 曹宁[1] 魏小曼[2] 郭东伟[1] 齐浩[2] 刘秋晨[1] 

机构地区：[1]黑龙江八一农垦大学生命技术学院,黑龙江大庆163319 [2]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《江西农业学报》2013年第3期88-92,共5页Acta Agriculturae Jiangxi

基　　金：黑龙江省教育厅科学技术研究项目(12511366)

摘　　要：目的:通过生物信息学及蛋白分析软件等,寻找ETEC K88、K99菌毛蛋白的抗原表位。方法:通过生物信息学技术、DNAStar Protean蛋白分析软件及网络共享软件对ETEC K88、K99菌毛蛋白抗原性相关的参数进行预测分析,从而设计ETEC K88、K99菌毛蛋白的抗原表位多肽;采用腹腔注射的方式将合成的抗原表位多肽免疫小鼠,用ELISA法检测血清中产生的抗体,并对免疫小鼠进行了攻毒试验。结果:多肽免疫小鼠后均能诱导抗体产生,攻毒试验表明合成肽对小鼠具有保护作用。结论:成功预测了抗原表位,合成的表位多肽具有较好的抗原性。In order to investigate the epitopes of ETEC K88 and K99 fimbrial protein, we designed and synthesized the epitope peptides of ETEC K88 and K99 fimbrial protein by bioinformatical and structural analysis software. The epitope peptides of ETEC K88 and K99 fimbrial protein were designed and synthesized after predicting and analyzing the relational parameters of the antigeni- city of＇~C K88 and K99 fimbrial protein with bioinformatics, DNAStar Protean and online sharing software. C57BL/6 mice were immunized with these synthetic peptides, the antibodies in serum were measured by ELISA, and the challenge test was implemented on the immune mice. The results showed that two synthetic peptides could induce high titers of specific antibodies after immunologi- cal reaction. The resuhs of challenge test with C83912 or C83902 showed that protection activity was found in the mice immunized with these synthetic peptides. It was concluded that the epitopes were successfully predicted, and the synthetic epitope peptides showed high antigenicity.

关 键 词：ETEC K99菌毛蛋白 ETEC K88菌毛蛋白 抗原表位 合成肽 

分 类 号：S852.61[农业科学—基础兽医学]