黄羽肉鸡IGF-1基因与饲料报酬性状的关系研究  

The study on the relations of chicken IGF-1 gene and feed conversion traits

作　　者：张燕 黄晓灵[2] 季从亮 彭志军 张德祥[1,2] 

机构地区：[1]广东温氏南方家禽育种有限公司,广东新兴527439 [2]华南农业大学动物科学学院,广州510642

出　　处：《黑龙江畜牧兽医》2013年第4期37-39,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家肉鸡产业技术体系专项项目(CARS-42-Z17)

摘　　要：为了探讨胰岛素样生长因子-1(IGF-1)基因作为黄羽肉鸡饲料报酬分子标记的可行性,试验采用PCR-RFLP技术对394只黄羽矮小型肉鸡IGF-1基因的5'侧翼区进行遗传多态性分析。结果表明:IGF-1基因的5'侧翼区PCR扩增产物经HinfⅠ酶切后出现AA、AC和CC 3种基因型;经χ2检验,群体在该位点上处于Hardy-Weinberg平衡状态(P>0.05);不同基因型的最小二乘平均值分析发现,纯合基因型之间饲料报酬差异显著或极显著(P<0.05或P<0.01),AA基因型个体显著优于CC基因型个体,A为优势等位基因。To investigate the feasibility of IGF - 1 gene as molecular marker of feed conversion ratio in yellow chicken, PCR - RFLP technology was used to analyze the genetic polymorphisms at 5 ＇ flanking region of the IGF - 1 gene from 394 yellow - feathered dwarf broilers. The results showed that three genotypes of AA, AC and CC were found when the PCR products at 5＇ flanking region of the IGF -1 gene were digested by Hinf I enzyme. The X2 test showed that the dwarf population were in Hardy -Weinberg equilibrium in the locus （P 〉 0. 05 ）. A least squares mean analysis for different genotypes showed that there was a significant or extremely significant difference in the feed conversion ratio between the homozygous genotypes （ P 〈 0.05 or P 〈 0.01 ）, in which genotype AA was significantly lower than genotype CC, and A was the dominant allele.

关 键 词：黄羽肉鸡 胰岛素样生长因子-1(IGF-1) PCR—RFLP 饲料报酬 

分 类 号：S831[农业科学—畜牧学]